实验性白癜风C57BL/6小鼠中的氧化应激
Oxidative stress in experimental vitiligo C57BL/6 mice.
作者信息
Jalel Akrem, Yassine Mrabet, Hamdaoui Mohamed Hédi
机构信息
Unité de Recherche sur les Composés antioxydants, Stress Oxydant, Eléments Traces et Maladies Métaboliques, Ecole Supérieure des Sciences et Techniques de la Santé de Tunis.
出版信息
Indian J Dermatol. 2009 Jul;54(3):221-4. doi: 10.4103/0019-5154.55628.
AIM
To evaluate whether oxidative stress is implicated in melanocyte damage in vitiligo.
BACKGROUND
Vitiligo is a complex disorder characterized by gradually enlarging areas of depigmentation. A new unifying hypothesis for the etiology of this pigment disorder is proposed, in which we postulate that the final destruction of melanocytes in vitiligo results from a cascade of reactions initiated by a disregulation of melanogenesis, as the result of a breakdown in free radical defense.
METHODS
We evaluated 18 vitiligo mice and 12 controls that were age matched. Parameters of oxidative stress such as catalase (CAT), superoxide dismutase (SOD), and plasma malondialdehyde (MDA) were measured by spectrophotometry.
RESULTS
MDA levels in vitiligo mice were significantly higher than in controls (P < 0.001). CAT, SOD, and glutathione peroxidase (GPx) activities in mice were significantly lower than controls (P < 0.05 and P < 0.001, respectively).
CONCLUSION
Our results confirmed that oxidative stress plays an important role in the pathogenesis of vitiligo. Melanocyte damage in vitiligo might be linked to generalized oxidative stress. This study is the first report on antioxidant parameters in experimental vitiligo mice.
目的
评估氧化应激是否与白癜风中黑素细胞损伤有关。
背景
白癜风是一种复杂的疾病，其特征为色素脱失区域逐渐扩大。我们提出了一种关于这种色素紊乱病因的新统一假说，即我们假定白癜风中黑素细胞的最终破坏是由黑素生成失调引发的一系列反应导致的，而黑素生成失调是自由基防御功能崩溃的结果。
方法
我们评估了18只白癜风小鼠和12只年龄匹配的对照小鼠。通过分光光度法测量氧化应激参数，如过氧化氢酶（CAT）、超氧化物歧化酶（SOD）和血浆丙二醛（MDA）。
结果
白癜风小鼠的MDA水平显著高于对照组（P < 0.001）。小鼠的CAT、SOD和谷胱甘肽过氧化物酶（GPx）活性显著低于对照组（分别为P < 0.05和P < 0.001）。
结论
我们的结果证实氧化应激在白癜风发病机制中起重要作用。白癜风中的黑素细胞损伤可能与全身性氧化应激有关。本研究是关于实验性白癜风小鼠抗氧化参数的首份报告。
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