Jeganathan N S, Kannan K, Manavalan R, Vasanthi Hannah R
Department of Pharmacy, Annamalai University, Annamalai Nagar-608 002, Tamil Nadu, India.
Afr J Tradit Complement Altern Med. 2008 Jan 22;5(2):131-40. doi: 10.4314/ajtcam.v5i2.31264.
Amukkara curanam, a Siddha formulation, currently used in all types of gastric disorders, rheumatic pain, insomnia and sexual insufficiency, was investigated for the estimation of the marker compounds, withaferine A and piperine contents in a prepared standard formulation and a commercial formulation by using HPTLC method of analysis. The two formulations were subjected to methanol, ethyl acetate and chloroform extractions by using Soxhhlet apparatus The chromatogram was developed using chloroform: methanol (8.5:1.5 v/v) and toluene: ethyl acetate (7:3 v/v) as mobile phases for the estimation of withferine A and piperine respectively. The detection and quantification were performed at a wavelength of 220 nm for withaferine A and 254 nm for piperine. The linear regression analysis of calibration plots of withferine A and piperine exhibited linear relationship in the range of 5-15 microg and 50-150 ng respectively, while the % recovery was found to be 94.52% w/w of withaferine A and 98.73% w/w of piperine, thus proving the accuracy and precision of the analysis. Methanol and ethyl acetate were found to be the suitable solvents for the extraction of withaferin A and piperine respectively. The withaferine A content in standard formulation was found to be much higher in all the three extracts than that of the commercial sample. However, the piperine content in all the three extracts of standard formulation was slightly lower than the respective extracts of commercial formulation. The proposed HPTLC method was found to be rapid, simple and accurate for quantitative estimation of withferine A and piperine in different formulation extracts.
阿穆卡拉丸剂是一种悉达配方药物，目前用于治疗各类胃部疾病、风湿性疼痛、失眠和性功能不全。本研究采用高效薄层色谱分析法，对一种制备好的标准制剂和一种市售制剂中的标记化合物——睡茄内酯A和胡椒碱的含量进行了测定。使用索氏提取器对这两种制剂分别进行甲醇、乙酸乙酯和氯仿提取。分别以氯仿：甲醇（8.5:1.5 v/v）和甲苯：乙酸乙酯（7:3 v/v）为流动相展开色谱图，用于测定睡茄内酯A和胡椒碱。在220 nm波长下检测和定量睡茄内酯A，在254 nm波长下检测和定量胡椒碱。睡茄内酯A和胡椒碱校准曲线的线性回归分析显示，线性关系范围分别为5 - 15微克和50 - 150纳克，而睡茄内酯A的回收率为94.52% w/w，胡椒碱的回收率为98.73% w/w，从而证明了分析的准确性和精密度。发现甲醇和乙酸乙酯分别是提取睡茄内酯A和胡椒碱的合适溶剂。标准制剂中所有三种提取物中的睡茄内酯A含量均远高于市售样品。然而，标准制剂所有三种提取物中的胡椒碱含量略低于市售制剂的相应提取物。结果表明，所提出的高效薄层色谱法对于不同制剂提取物中睡茄内酯A和胡椒碱的定量测定快速、简便且准确。
