Hazra Alok K, Chakraborty Banti, Mitra Achintya, Sur Tapas Kumar
Division of Pharmaceutical Chemistry, R.K.M.A Quality Testing Laboratory, Kolkata, 700103, India.
National Research Institute of Ayurvedic Drug Development, CCRAS, Kolkata, 700091, India.
J Ayurveda Integr Med. 2019 Oct-Dec;10(4):248-254. doi: 10.1016/j.jaim.2017.07.006. Epub 2018 Oct 11.
Trikatu, Sitopaladi, Hingavastaka, Avipattikara, Sringyadi and Talisadya are very popular Ayurvedic (churna) medicines practiced in India; however, unfortunately, they possess several quality control issues.
The aim of this study was to find out a simple, accurate and sensitive HPTLC method for the detection and quantification of marker molecule, piperine (alkaloid) on these Ayurvedic formulations for standardization.
Methanolic extraction (reflux) was performed from the above six churnas as well as three single ingredients Piper longum (pipul), Piper nigrum (marich) and Piper chaba (chai). HPTLC was done using piperine as a standard. The mobile phase was a mixture of toluene-ethyl acetate (7:3, v/v) and detection at 342λ.
The R was detected at 0.39. Piperine was quantified in all samples. P. nigrum showed higher piperine than P. longum and P. chaba. The maximum piperine was noted in Hingavastaka churna and followed by Sringyadi churna, Sitopaladi churna, Talisadya churna, Trikatu churna and Avipattikara churna.
This method can be successfully employed for standardization and quantitative analysis of piperine in Ayurvedic formulations (churnas) and also be helpful to clinicians and pharmacists to draw significant role of piperine present in all these samples.
三卡图、西托帕拉迪、辛加瓦斯塔卡、阿维帕蒂卡拉、斯灵亚迪和塔利萨迪亚是印度非常流行的阿育吠陀（药粉）药物；然而，不幸的是，它们存在几个质量控制问题。
本研究的目的是找出一种简单、准确且灵敏的高效薄层色谱法，用于检测和定量这些阿育吠陀制剂中的标记分子胡椒碱（生物碱），以实现标准化。
对上述六种药粉以及三种单一成分荜茇、胡椒和假蒌进行甲醇提取（回流）。以胡椒碱为标准品进行高效薄层色谱分析。流动相为甲苯 - 乙酸乙酯（7:3，v/v）混合液，检测波长为342λ。
Rf值为0.39。对所有样品中的胡椒碱进行了定量。胡椒中的胡椒碱含量高于荜茇和假蒌。辛加瓦斯塔卡药粉中胡椒碱含量最高，其次是斯灵亚迪药粉、西托帕拉迪药粉、塔利萨迪亚药粉、三卡图药粉和阿维帕蒂卡拉药粉。
该方法可成功用于阿育吠陀制剂（药粉）中胡椒碱的标准化和定量分析，也有助于临床医生和药剂师了解所有这些样品中胡椒碱的重要作用。
