Habbu P V, Shastry R A, Mahadevan K M, Joshi Hanumanthachar, Das S K
Department of Pharmacognosy and Phytochemistry S.E.T's College of Pharmacy, S.R.Nagar, Dharwad, Karnataka, India.
Afr J Tradit Complement Altern Med. 2008 Jan 22;5(2):158-64. doi: 10.4314/ajtcam.v5i2.31268.
The present study has been designed to evaluate the liver protective and in-vivo antioxidant role of Ethanolic extract (EtAS) and Ethyl acetate extract (EAAS) of roots of Argyreia speciosa, an important 'rasayana' herb in Indian System of medicine, in CCl(4)-induced hepatotoxicity and oxidative stress in rats. Animals were treated with EtAS and EAAS at doses of 200 mg and 400 mg/kg body weight p.o. along with CCl(4) (0.7 ml/kg in olive oil, 1:1 v/v i.p. on every alternate days) for seven days. Serum biochemical parameters such as SGOT, SGPT, ALP, cholesterol, total and direct bilirubin were determined. Antioxidant status in liver was determined by measuring the activities of Super oxide dismutase (SOD), catalase and peroxidase. Histopathological study of isolated liver specimens was also carried out to know the protection offered by the extracts. There was a significant rise in the levels of serum GOT, GPT, and ALP and other biochemical parameters, decrease in the levels of SOD, catalase and peroxidase after administration of CCl(4). Suspensions of EtAS and EAAS (200 and 400 mg/kg) successfully prevented the alterations of these effects in rats (p< 0.001). Histopathological examination demonstrated that CCl(4) treated group induces ballooning degeneration and centrilobular necrosis. Groups treated with EtAS and EAAS showed recovery on ballooning degeneration and centrolobular bridging necrosis was occasionally present. Data also showed that these extracts possessed strong antioxidant activity, and were comparable to Silymarin, a well known liver protecting herbal formulation.
本研究旨在评估印度传统医学中一种重要的“rasayana”草药——银背藤根的乙醇提取物（EtAS）和乙酸乙酯提取物（EAAS）对四氯化碳诱导的大鼠肝毒性和氧化应激的肝脏保护作用及体内抗氧化作用。动物分别经口给予200 mg和400 mg/kg体重的EtAS和EAAS，同时腹腔注射四氯化碳（0.7 ml/kg溶于橄榄油，1:1 v/v，隔日一次），持续7天。测定血清生化参数，如谷草转氨酶（SGOT）、谷丙转氨酶（SGPT）、碱性磷酸酶（ALP）、胆固醇、总胆红素和直接胆红素。通过测量超氧化物歧化酶（SOD）、过氧化氢酶和过氧化物酶的活性来确定肝脏的抗氧化状态。还对分离的肝脏标本进行了组织病理学研究，以了解提取物提供的保护作用。给予四氯化碳后，血清谷草转氨酶、谷丙转氨酶和碱性磷酸酶水平及其他生化参数显著升高，超氧化物歧化酶、过氧化氢酶和过氧化物酶水平降低。EtAS和EAAS（200和400 mg/kg）混悬液成功预防了大鼠这些效应的改变（p<0.001）。组织病理学检查表明，四氯化碳处理组诱导了气球样变性和小叶中心坏死。EtAS和EAAS处理组显示气球样变性有所恢复，偶尔出现小叶中心桥接坏死。数据还表明，这些提取物具有很强的抗氧化活性，与著名的肝脏保护草药制剂水飞蓟素相当。
