Menkem Brice, Vemo Bertin Narcisse, Tsambou Megnimeza Martine Astride, Fonou Tadiesse Lavoisier, Dongmo Nguedia Arius Baulland, Boufack Judith Laure, Chongsi Margaret Mary Momo, Kenfack Augustave
Department of Animal Science, Faculty of Agronomy and Agricultural Sciences, University of Dschang, Dschang, Cameroon.
Department of Animal Science, Faculty of Agriculture and Veterinary Medicine, University of Buea, Buea, Cameroon.
Front Vet Sci. 2021 Oct 12;8:736836. doi: 10.3389/fvets.2021.736836. eCollection 2021.
This study aimed at evaluating the therapeutic effects of ethanolic extract of (EEPLM) on the reproductive parameters of male Guinea pigs exposed to cadmium chloride. Thirty-six male guinea pigs were randomly assigned to six treatment groups (with six animals per group). Group 1 (DW) received distilled water orally; group 2 (Cd), negative control, was treated with cadmium chloride at a dose of 26.25 mg/kg body weight (bw); while group 3 (VitC), positive control, was given 26.25 mg of cadmium chloride/kg bw and 100 mg of vitamin C, and groups 4, 5, and 6 were treated, respectively, with EEPLM at doses of 50, 100, and 200 mg/kg bw in addition to cadmium chloride (26.25 mg/kg bw). After 90 days, all animals were sacrificed, and data related to reproduction, toxicity, and oxidative stress were collected. Results revealed a significant decrease ( < 0.05) of serum levels of creatinine, urea, alanine, and aspartate amino transferases in guinea pigs treated with cadmium chloride and EEPLM compared to the negative control group (Cd). The weight of the bulbo-urethral gland was significantly ( < 0.05) decreased in animals exposed to cadmium chloride and treated with vitamin C or EEPLM compared to the negative control (Cd). Guinea pigs orally receiving cadmium chloride and EEPLM showed significantly ( < 0.05) increased motility, sperm count, spermatozoa with entire plasma membrane, and percentage of normal spermatozoa with reference to the negative control (Cd). The serum level of testosterone increased insignificantly ( > 0.05) in animals given cadmium and EEPLM compared to the negative control (Cd). Animals co-administered cadmium chloride and EEPLM recorded a significantly ( < 0.05) reduced level of MDA, activities of SOD, and total peroxidases compared to the group that received cadmium chloride (Cd) only. In conclusion, cadmium chloride induced reproductive impairments by generating oxidative stress. However, the administration of EEPLM can mitigate these adverse effects due to its antioxidant properties.
本研究旨在评估酸浆叶下珠乙醇提取物（EEPLM）对暴露于氯化镉的雄性豚鼠生殖参数的治疗效果。36只雄性豚鼠被随机分为6个治疗组（每组6只动物）。第1组（DW）口服蒸馏水；第2组（Cd）为阴性对照组，用剂量为26.25毫克/千克体重（bw）的氯化镉处理；而第3组（VitC）为阳性对照组，给予26.25毫克/千克bw的氯化镉和100毫克维生素C，第4、5和6组除了给予26.25毫克/千克bw的氯化镉外，还分别用50、100和200毫克/千克bw的EEPLM处理。90天后，处死所有动物，并收集与生殖、毒性和氧化应激相关的数据。结果显示，与阴性对照组（Cd）相比，用氯化镉和EEPLM处理的豚鼠血清肌酐、尿素、丙氨酸和天冬氨酸氨基转移酶水平显著降低（P<0.05）。与阴性对照组（Cd）相比，暴露于氯化镉并用维生素C或EEPLM处理的动物球海绵体腺重量显著降低（P<0.05）。口服氯化镉和EEPLM的豚鼠与阴性对照组（Cd）相比，精子活力、精子计数、具有完整质膜的精子以及正常精子百分比显著增加（P<0.05）。与阴性对照组（Cd）相比，给予镉和EEPLM的动物血清睾酮水平无显著增加（P>0.05）。与仅接受氯化镉（Cd）的组相比，同时给予氯化镉和EEPLM的动物丙二醛水平、超氧化物歧化酶活性和总过氧化物酶显著降低（P<0.05）。总之，氯化镉通过产生氧化应激诱导生殖损伤。然而，EEPLM的给药因其抗氧化特性可减轻这些不良反应。
