Singh B, Saxena A K, Chandan B K, Agarwal S G, Anand K K
Division of Pharmacology, Regional Research Laboratory, Jammu Tawi-180 001.
Indian J Physiol Pharmacol. 2001 Oct;45(4):435-41.
The alcoholic extract of fresh leaves of the plant Eclipta alba (Ea), previously reported for is hepatoprotective activity was fractionated into three parts to chemically identify the most potent bioactive fraction. The hepatoprotective potential of the fraction prepared from extract was studied in vivo in rats and mice against carbon tetrachloride induced hepatotoxicity. The hepatoprotective activity was determined on the basis of their effects on parameters like hexobarbitone sleep time, zoxazolamine paralysis time, bromosulphaline clearance, serum transaminases and serum bilirubin. Fraction EaII (10-80 mg/kg, p.o.) containing coumestan wedelolactone and desmethylwedelolactone as major components with apigenin, luteolin, 4-hydroxybenzoic acid and protocateuic acid as minor constituents exhibited maximum hepatoprotective activity and is the active fraction for hepatoprotective activity of Eclipta alba leave. The acute toxicity studies have shown that like Ea, Fraction EaII also high safety margin.
此前报道具有保肝活性的植物旱莲草（Eclipta alba，Ea）鲜叶的乙醇提取物被分成三个部分，以化学鉴定出最有效的生物活性部分。在大鼠和小鼠体内研究了从提取物制备的该部分对四氯化碳诱导的肝毒性的保肝潜力。根据其对诸如己巴比妥睡眠时间、唑沙宗麻痹时间、溴磺酚酞清除率、血清转氨酶和血清胆红素等参数的影响来确定保肝活性。含有主要成分香豆雌酚和去甲基香豆雌酚以及次要成分芹菜素、木犀草素、4-羟基苯甲酸和原儿茶酸的EaII部分（10 - 80毫克/千克，口服）表现出最大的保肝活性，是旱莲草叶保肝活性的活性部分。急性毒性研究表明，与Ea一样，EaII部分也有很高的安全系数。
