Adhvaryu Meghna R, Reddy Narshimha, Parabia Minoo H
Bapalal Vaidya Botanical Research Centre, Dept. of Biosciences, Veer Narmad South Gujarat University, Surat-395007, India.
Afr J Tradit Complement Altern Med. 2008 Jun 18;5(4):409-18. doi: 10.4314/ajtcam.v5i4.31297.
The anti-tumor activity and chemopreventive potential of four Ayurvedic herbs viz. Curcuma longa L., Ocimum sanctum L., Tinospora cordifolia (Wild) Miers ex Hook.f & Thomas and Zizyphus mauritiana Lam. were evaluated using Dalton Lymphoma ascites (DLA) tumor model in Swiss Albino mice. The outcome was assessed using survival time, peritoneal ascitic fluid (Tumor volume) and hematological indices as parameters. Animals were divided into five groups (n = 6) viz. one DLA control and four Herb + DLA treated groups. All the four herb + DLA groups were pre-treated with respective herbs for 7 days and hematological indices were measured for entire five groups. On day-8 animals were inoculated with 1x10(6) DLA cells i.p., and Herb + DLA groups were continued with oral herbal treatment for 21-days. Hematological parameters and tumor volume were assessed to find the effects of herbs. Short term in vitro cytotoxicity was determined by Trypan Blue exclusion method and LDH leakage assay using different concentrations of herbal extracts and 5-FU as a positive control and IC(50) for each herbal extract and 5-FU were determined. Oral administration of crude herb increased the survival time and decreased the peritoneal ascitic fluid content significantly. Hb, RBCs and total WBC which were altered by DLA inoculation were restored significantly by all the herbs except O. sanctum. All the four herbs showed in vitro cytotoxic activity against DLA cell-line. Moreover inter group comparison of all the four herbs for anti-tumor activity showed efficacy in the following order--T. cordifolia > Z. mauritiana > or = C. longa > O. sanctum respectively.
使用瑞士白化小鼠的道尔顿淋巴瘤腹水（DLA）肿瘤模型，对四种阿育吠陀草药，即姜黄、圣罗勒、心叶地锦和毛叶枣的抗肿瘤活性和化学预防潜力进行了评估。以生存时间、腹腔腹水（肿瘤体积）和血液学指标为参数评估结果。将动物分为五组（n = 6），即一个DLA对照组和四个草药+DLA治疗组。所有四个草药+DLA组均用各自的草药预处理7天，并测量所有五组的血液学指标。在第8天，给动物腹腔注射1x10(6)个DLA细胞，草药+DLA组继续口服草药治疗21天。评估血液学参数和肿瘤体积以发现草药的作用。通过台盼蓝排斥法和乳酸脱氢酶泄漏试验，使用不同浓度的草药提取物和5-氟尿嘧啶作为阳性对照，测定短期体外细胞毒性，并确定每种草药提取物和5-氟尿嘧啶的半数抑制浓度（IC(50)）。口服粗草药可显著延长生存时间并降低腹腔腹水含量。除圣罗勒外，所有草药均能显著恢复因接种DLA而改变的血红蛋白、红细胞和总白细胞水平。所有四种草药均对DLA细胞系显示出体外细胞毒性活性。此外，对所有四种草药的抗肿瘤活性进行组间比较，结果显示其疗效顺序如下：心叶地锦>毛叶枣>或=姜黄>圣罗勒。
