Departamento de Química Física, Universidade de Santiago de Compostela, Lugo, Spain.
Int J Mol Sci. 2010 Jan 12;11(1):173-88. doi: 10.3390/ijms11010173.
The host-guest complexation between an Alexa 488 labelled adamantane derivative and beta-cyclodextrin is studied by Fluorescence Correlation Spectroscopy (FCS). A 1:1 complex stoichiometry and a high association equilibrium constant of K = 5.2 x 10(4) M(-1) are obtained in aqueous solution at 25 degrees C and pH = 6. The necessary experimental conditions are discussed. FCS proves to be an excellent method for the determination of stoichiometry and association equilibrium constant of this type of complexes, where both host and guest are nonfluorescent and which are therefore not easily amenable to standard fluorescence spectroscopic methods.
荧光相关光谱（FCS）研究了 Alexa 488 标记的金刚烷衍生物与β-环糊精之间的主体-客体络合作用。在 25°C 和 pH = 6 的水溶液中，获得了 1:1 络合物化学计量比和高缔合平衡常数 K = 5.2 x 10(4) M(-1)。讨论了必要的实验条件。FCS 被证明是确定此类络合物的化学计量比和缔合平衡常数的一种极好方法，其中主体和客体均无荧光，因此不易采用标准荧光光谱方法。
