Yerevan Institute Plastpolymer, Arshakunyats Ave. 127, 0007, Yerevan, Armenia.
J Mater Sci Mater Med. 2010 May;21(5):1693-702. doi: 10.1007/s10856-010-4020-x. Epub 2010 Feb 17.
The aim of this study was to investigate hemocompatibility and cytotoxicity properties of synthetic polymer coatings containing various unsaturated carbonic acids with vinylacetate. Co-polymers of vinylacetate and crotonic acid (CA), maleic acid (MA), and itaconic acid (IA) were made. The materials were characterized in terms of their adhesion to metal supports (titanium and stainless steel) as well as hemocompatibility (% hemolysis, wettability, erythrocyte aggregation, hemoglobin content, thrombocyte count and lipid peroxidation levels) and cytotoxicity (human endothelial cell activity in vitro and chromosome aberrations, bone marrow proliferation and cell ploidy in rats). Co-polymers of unsaturated carbonic acids with vinylacetate exhibited good hemocompatibility properties, as opposed to vinylacetate homopolymer for which substantial levels of hemolysis were observed. By coating the metal supports with co-polymers the cytotoxic effects associated with the bare metal samples were markedly reduced. MA samples showed excellent hemocompatibility and no cytotoxicity, yet they lacked good adhesion properties to metal substrate, probably due to high water content. CA samples, having the highest density of carboxylic groups among the samples under investigation, showed increased bone marrow proliferation activity and cell ploidy in rats, as compared to controls. The most promising results in the present study were obtained for the samples with IA, which showed good adhesion to metal substrates, good hemocompatibility and low cytotoxicity. Thus, co-polymers of vinylacetate and IA rich in carboxylic groups are promising materials for the design of novel drug-eluting stents.
本研究旨在探讨含有不同不饱和碳酸的合成聚合物涂层与醋酸乙烯酯的血液相容性和细胞毒性特性。制备了醋酸乙烯酯与巴豆酸（CA）、马来酸（MA）和衣康酸（IA）的共聚物。从材料对金属（钛和不锈钢）基底的附着力以及血液相容性（%溶血、润湿性、红细胞聚集、血红蛋白含量、血小板计数和脂质过氧化水平）和细胞毒性（体外人内皮细胞活性和染色体畸变、大鼠骨髓增殖和细胞倍性）等方面对材料进行了表征。与观察到大量溶血的醋酸乙烯酯均聚物相比，含有不饱和碳酸的醋酸乙烯酯共聚物表现出良好的血液相容性。通过将共聚物涂覆在金属基底上，显著降低了与裸金属样品相关的细胞毒性作用。MA 样品表现出极好的血液相容性和无细胞毒性，但由于高含水量，它们对金属基底的附着力较差。CA 样品在研究的样品中具有最高密度的羧基，与对照组相比，在大鼠中显示出增加的骨髓增殖活性和细胞倍性。在本研究中，最有前途的结果是 IA 样品获得的，其对金属基底具有良好的附着力、良好的血液相容性和低细胞毒性。因此，富含羧基的醋酸乙烯酯共聚物是设计新型药物洗脱支架的有前途的材料。
