Department of Chemical Engineering, Faculty of Science, University of Vigo (Campus Ourense), As Lagoas, Ourense, Spain.
J Agric Food Chem. 2010 Mar 24;58(6):3632-41. doi: 10.1021/jf904508g.
Oligosaccharides produced by hydrothermal processing of rice husks (xylooligosaccharides and glucooligosaccharides) were refined by membrane processing (operating in diafiltration and concentration modes), subjected to xylanase treatment to reduce the average molar mass, and subjected to further purification by ultrafiltration (operating in concentration mode) and ion exchange. The purified products were assayed for composition, molar mass distribution and structural characterization by HPLC, HPAEC-PAD, HPSEC, MALDI-TOF-MS and NMR ((1)H and (13)C). The fermentability of the purified product by fecal inocula was assessed on the basis of the time courses of pH and oligosaccharide concentrations. Succinate, lactate, formiate, acetate, propionate and butyrate were the major products resulting from fermentation experiments.
通过水热处理稻壳得到的低聚糖（木低聚糖和葡低聚糖）经过膜处理（在渗滤和浓缩模式下运行）进行精制，用木聚糖酶处理以降低平均摩尔质量，并通过超滤（在浓缩模式下运行）和离子交换进一步纯化。通过 HPLC、HPAEC-PAD、HPSEC、MALDI-TOF-MS 和 NMR（（1）H 和（13）C）对纯化产物进行组成、摩尔质量分布和结构表征分析。根据 pH 值和低聚糖浓度随时间的变化，评估粪便接种物对纯化产物的发酵能力。发酵实验的主要产物是琥珀酸、乳酸、甲酸、乙酸、丙酸和丁酸。
