Yang Shuhua, Gong Ping, Pan Jianwen, Wang Nan, Tong Jingjing, Wang Mingyang, Long Miao, Li Peng, He Jianbin
Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China.
Institute of Animal Husbandry Quality Standards, Xinjiang Academy of Animal Science, Urumqi 830000, China.
Microorganisms. 2019 Aug 16;7(8):266. doi: 10.3390/microorganisms7080266.
Zearalenone (ZEA) contamination is a very serious problem around the world as it can induce reproductive disorders in animals and affect the health of humans. Therefore, reducing the damage it causes to humans and animals is a current focus of research. In this study, we assess the removing capacity of xy46 towards ZEA and investigate the mechanism responsible for its action, thus confirming if it can alleviate ZEA toxicity to the reproductive systems of male mice. Our results show that the rate at which the strain removes ZEA is as high as 89.2% in 48 h when the concentration of ZEA is 4 μg/mL in the liquid medium. Heat and acid treatment significantly enhanced the ability of the bacteria to remove ZEA. The animal experiments results show that the oral administration of xy46 to mice (0.2 mL daily at a concentration of 10 CFU/mL for 28 days) significantly reduces the degree of testicular pathomorphological changes and apoptosis induced by ZEA when the mice are intragastric administration with 40 mg/kg ZEA daily for 28 days. Moreover, oral administration of xy46 enhances the decrease in the testosterone level and improves the oxidative stress injury induced by ZEA. Furthermore, oral administration of xy46 reverts the expression of these genes and proteins in the testicular tissues of the mice involved in the blood-testis barrier and apoptosis (e.g., Vim, caspase 12, Cldn11, N-cad, Bax, and Bcl-2). However, xy46 cannot significantly revert in some of these evaluated parameters, especially in sperm quantity and quality when the mice were given 70 mg/kg ZEA daily for 28 days. In conclusion, our results suggest that the strain xy46 can efficiently remove ZEA from the liquid medium, the mechanism responsible for its action is absorption, and it can alleviate the toxicity of ZEA to the reproductive systems of male mice when the mice are given 40 mg/kg ZEA daily, However, it cannot completely alleviate the reproductive toxicity of higher dosage of zearalenone through its ability to adsorb ZEA.
玉米赤霉烯酮（ZEA）污染是一个全球性的严重问题，因为它可诱发动物生殖紊乱并影响人类健康。因此，减少其对人类和动物造成的损害是当前的研究重点。在本研究中，我们评估了xy46对ZEA的去除能力，并探究其作用机制，从而确定它是否能减轻ZEA对雄性小鼠生殖系统的毒性。我们的结果表明，当液体培养基中ZEA浓度为4μg/mL时，该菌株在48小时内对ZEA的去除率高达89.2%。加热和酸处理显著增强了该细菌去除ZEA的能力。动物实验结果表明，给小鼠口服xy46（每天0.2 mL，浓度为10 CFU/mL，共28天），当小鼠每天灌胃40 mg/kg ZEA，持续28天时，可显著降低ZEA诱导的睾丸病理形态学变化程度和细胞凋亡。此外，口服xy46可增强睾酮水平的降低，并改善ZEA诱导的氧化应激损伤。此外，口服xy46可使参与血睾屏障和细胞凋亡的小鼠睾丸组织中这些基因和蛋白质的表达恢复正常（如波形蛋白、半胱天冬酶12、紧密连接蛋白11、N-钙黏蛋白、 Bax和Bcl-2）。然而，当小鼠每天给予70 mg/kg ZEA，持续28天时，xy46不能使其中一些评估参数显著恢复正常，尤其是精子数量和质量。总之，我们的结果表明，菌株xy46能有效从液体培养基中去除ZEA，其作用机制是吸附，当小鼠每天给予40 mg/kg ZEA时，它可减轻ZEA对雄性小鼠生殖系统的毒性，然而，它不能通过吸附ZEA的能力完全减轻高剂量玉米赤霉烯酮的生殖毒性。
