Departamento de Protección Vegetal, INIA, Madrid, Spain.
J Appl Microbiol. 2010 Aug;109(2):583-593. doi: 10.1111/j.1365-2672.2010.04681.x. Epub 2010 Jan 22.
To find a formulation of Epicoccum nigrum conidia that enhances its adhesion to peach surfaces and improves its biocontrol efficacy against brown rot caused by Monilinia laxa.
The stickers, glycerol, sodium alginate and methylcellulose; the desiccants, silica powder and talc; and a commercial adhesive (NU FILM 17) were added at two different points during the production of an E. nigrum conidial formulation to improve conidial adhesion to peach surfaces. Conidial adhesion levels were determined from the number of E. nigrum conidia that adhered to glass slides or peach surfaces and conidial viability of adherent E. nigrum conidia was determined from the number of colony-forming units of glass or peach-adherent E. nigrum that grew on Petri dishes that contained potato dextrose agar. Compared to dried E. nigrum conidia without additives, the adhesion and viability of adherent E. nigrum conidia to peach surfaces were enhanced when either 1.25% sodium alginate or 2.5% methylcellulose was added to the conidial mass after fluid-bed drying, and when 2.5% methylcellulose was added to the conidial mass after its production and before fluid-bed drying. Epicoccum nigrum conidial formulations with 2.5% methylcellulose were more effective than dried E. nigrum conidia without additives in reducing the incidence of brown rot in peaches caused by M. laxa.
When 2.5% methylcellulose is incorporated into an E. nigrum conidial formulation, the adhesion of E. nigrum conidia to peach surfaces improves and results in efficacious biocontrol of brown rot.
A new improved formulation of a biocontrol agent has been developed to improve the control of M. laxa on peaches.
找到一种增强欧洲地霉菌分生孢子附着在桃表面的制剂，并提高其对由密枝木霉菌引起的褐腐病的生物防治效果。
在生产欧洲地霉菌分生孢子制剂的两个不同点添加了贴纸、甘油、海藻酸钠和甲基纤维素；干燥剂、硅粉和滑石粉；以及一种商业粘合剂（NU FILM 17），以提高分生孢子对桃表面的附着。通过计算附着在载玻片或桃表面上的欧洲地霉菌分生孢子的数量来确定分生孢子的附着水平，通过计算在含有马铃薯葡萄糖琼脂的培养皿上生长的玻璃或桃附着的欧洲地霉菌的菌落形成单位数来确定附着的欧洲地霉菌分生孢子的活力。与未添加添加剂的干燥欧洲地霉菌分生孢子相比，在流化床干燥后将 1.25%的海藻酸钠或 2.5%的甲基纤维素添加到分生孢子团中，以及在生产后并在流化床干燥之前将 2.5%的甲基纤维素添加到分生孢子团中时，分生孢子对桃表面的附着和活力都得到了增强。含有 2.5%甲基纤维素的欧洲地霉菌分生孢子制剂在减少由密枝木霉菌引起的桃褐腐病的发病率方面比未添加添加剂的干燥欧洲地霉菌分生孢子更有效。
当 2.5%甲基纤维素被纳入欧洲地霉菌分生孢子制剂中时，欧洲地霉菌分生孢子对桃表面的附着能力得到提高，并有效地控制了褐腐病。
开发了一种新的生物防治剂改良制剂，以改善对密枝木霉菌在桃上的控制。
