Kumari Ranjna, Kumar Vipul, Koul Bhupendra, Abul Farah Mohammad, Mishra Awdhesh Kumar
Department of Botany, Lovely Professional University, Phagwara, Punjab, India.
Department of Plant Pathology, School of Agriculture, Lovely Professional University, Phagwara, Punjab, India.
Front Microbiol. 2025 May 1;16:1583114. doi: 10.3389/fmicb.2025.1583114. eCollection 2025.
This study aims to identify and characterize four isolates using molecular techniques, Fourier transform infrared spectroscopy (FTIR), and volatile organic compounds (VOC) profiling.
The antagonistic activity of these isolates was assessed against f. sp. (FOC) and (SR) using a dual culture technique. The synergistic effect of (accession no. PP256488) combined with biochar (BC) was evaluated for plant growth enhancement and disease suppression. Four isolates (, and ) were identified through ITS region analysis, VOC profiling, and FTIR spectroscopy.
Molecular analysis confirmed their distinct identities, and GC-MS analysis revealed 37 VOCs out of 162 with antipathogenic properties. Unique FTIR peaks were recorded at 3271.96 cm for , 2800-2900 cm for , and 2850-2950 cm for both and . Scanning electron microscopy (SEM) analysis of revealed mycoparasitic structures, including hyphal coils, penetration holes, and appressoria, indicating effective pathogen interaction. The combined application of and biochar (T9) significantly enhanced root length (9.23 cm), plant height (26.03 cm), and root mass (43.33 g) in chickpea plants. Moreover, treatments (T9) and (T10) reduced the disease incidence in chickpeas, decreasing fusarium wilt by 27% and collar rot by 33%, respectively.
This sustainable approach exhibits the potential of combined application of and biochar which can enhance plant growth and reduce disease incidence, and improve food security.
本研究旨在利用分子技术、傅里叶变换红外光谱（FTIR）和挥发性有机化合物（VOC）分析来鉴定和表征四种分离物。
采用对峙培养技术评估这些分离物对尖孢镰刀菌（FOC）和立枯丝核菌（SR）的拮抗活性。评估了木霉菌（登录号PP256488）与生物炭（BC）组合对促进植物生长和抑制病害的协同效应。通过ITS区域分析、VOC分析和FTIR光谱鉴定了四种木霉菌分离物（、和）。
分子分析证实了它们的独特身份，气相色谱 - 质谱联用（GC - MS）分析显示162种中有37种具有抗病原特性的VOC。在处记录到独特的FTIR峰，在3271.96 cm处，在2800 - 2900 cm处，和在2850 - 2950 cm处。对的扫描电子显微镜（SEM）分析揭示了菌寄生结构，包括菌丝线圈、穿透孔和附着胞，表明有效的病原体相互作用。木霉菌和生物炭的组合应用（T9）显著增加了鹰嘴豆植株的根长（9.23 cm）、株高（26.03 cm）和根质量（43.33 g）。此外，处理（T9）和（T10）降低了鹰嘴豆的发病率，分别使枯萎病发病率降低27%，根腐病发病率降低33%。
这种可持续方法展示了木霉菌和生物炭组合应用的潜力，可促进植物生长、降低发病率并改善粮食安全。
