Laboratory of Animal Breeding and Genetics, Graduate School of Agriculture, Kyoto University, Sakyo, Kyoto, Japan.
Anim Sci J. 2009 Aug;80(4):486-9. doi: 10.1111/j.1740-0929.2009.00665.x.
Marbling, defined by the amount and distribution of intramuscular fat, is an economically important trait of beef cattle in Japan. The endothelial differentiation, sphingolipid G-protein-coupled receptor, 1 (EDG1) gene has been considered as a positional functional candidate for the gene responsible for marbling. We have recently reported that 2 single nucleotide polymorphisms (SNPs), c.-312A>G in the 5' untranslated region (UTR) and c.*446G>A in the 3' UTR in EDG1 were associated with marbling in Japanese Black beef cattle, but this was not functional and a causal mutation for marbling. In the present study, we detected 2 novel SNPs, referred to as g.1475435G>A and g.1471620G>T, in the 5' flanking region of the EDG1 between low-marbled and high-marbled steer groups, which were previously shown to have EDG1 expression differences in musculus longissimus muscle. The g.1475435G>A SNP seemed not to segregate in Japanese Black beef cattle. The g.1471620G>T SNP was associated with the predicted breeding value for beef marbling standard number by the analyses using Japanese Black beef cattle population. Based on these findings, we hypothesized that the g.1471620G>T SNP might have an impact on EDG1 expression and also marbling.
大理石花纹是日本肉牛的一个重要经济性状，由肌肉内脂肪的含量和分布决定。内皮分化、鞘脂类 G 蛋白偶联受体 1（EDG1）基因被认为是与大理石花纹有关的候选基因。我们最近报道称，EDG1 基因的 5'非翻译区（UTR）c.-312A>G 和 3'UTR c.*446G>A 这 2 个单核苷酸多态性（SNP）与日本黑牛肉牛的大理石花纹有关，但这并不是大理石花纹的功能和因果突变。在本研究中，我们在之前显示在 musculus longissimus 肌肉中 EDG1 表达存在差异的 EDG1 5'侧翼区检测到 2 个新的 SNP，分别称为 g.1475435G>A 和 g.1471620G>T，在低大理石花纹和高大理石花纹公牛组之间。g.1475435G>A SNP 似乎不在日本黑牛肉牛中分离。g.1471620G>T SNP 与使用日本黑牛肉牛群体进行的分析中牛肉大理石花纹标准数量的预测育种值相关。基于这些发现，我们假设 g.1471620G>T SNP 可能对 EDG1 表达和大理石花纹有影响。
