Kawaguchi Fuki, Tsuchimura Miyako, Oyama Kenji, Matsuhashi Tamako, Maruyama Shin, Mannen Hideyuki, Sasazaki Shinji
Laboratory of Animal Breeding and Genetics, Graduate School of Agricultural Science, Kobe University, Kobe, 657-8501, Japan.
Food Resources Education & Research Center, Kobe University, Kasai, 657-2103, Japan.
Arch Anim Breed. 2020 Jan 13;63(1):9-17. doi: 10.5194/aab-63-9-2020. eCollection 2020.
Carcass traits have been efficiently improved by recent selection using DNA markers in beef cattle. Additionally, DNA markers might have an effect on other traits such as fertility traits; therefore attention should also be paid to such pleiotropic effects. However, the effects of the markers on both carcass and fertility traits have never been evaluated in the same population, since they are generally measured in different populations. The objective in the current study was to discuss effectiveness of DNA markers developed for carcass traits through investigation of their effects on carcass and fertility traits in a population. We genotyped six markers V293A, g.841G>C, g.49066C>G, I442M, K232A, and g.1471620G>T in a Japanese Black cattle population ( ). To investigate their effects on carcass and fertility traits, we performed statistical analysis (ANOVA and the Tukey-Kramer honestly significant difference (HSD) test). In the results, three of six markers, V293A, I442M, and g.1471620G>T, were significantly associated with both carcass and fertility traits. Remarkably, the same allele for each marker had positive effects on both traits, suggesting that we would be able to simultaneously improve them using these markers in this population. However, previous studies reported that the effects of DNA markers could differ among populations. Therefore, it is necessary to confirm the effect of the marker in each population before it is used for improvement.
通过最近在肉牛中使用DNA标记进行选择，胴体性状得到了有效改善。此外，DNA标记可能对其他性状如繁殖性状有影响；因此，也应关注这种多效性效应。然而，由于标记对胴体和繁殖性状的影响通常在不同群体中进行测量，所以从未在同一群体中对其进行评估。本研究的目的是通过调查DNA标记在一个群体中对胴体和繁殖性状的影响，来探讨为胴体性状开发的DNA标记的有效性。我们对日本黑牛群体中的六个标记V293A、g.841G>C、g.49066C>G、I442M、K232A和g.1471620G>T进行了基因分型（ ）。为了研究它们对胴体和繁殖性状的影响，我们进行了统计分析（方差分析和Tukey-Kramer诚实显著差异（HSD）检验）。结果显示，六个标记中的三个，即V293A、I442M和g.1471620G>T，与胴体和繁殖性状均显著相关。值得注意的是，每个标记的相同等位基因对这两个性状都有积极影响，这表明在这个群体中我们能够使用这些标记同时改善这两个性状。然而之前的研究报道DNA标记的效应在不同群体间可能存在差异。因此，在将标记用于改良之前，有必要在每个群体中确认其效应。
