Tong B, Gao G Q, Muramatsu Y, Ohta T, Kose H, Li G P, Fatchiyah F, Yamada T
The Key Laboratory for Mammalian Reproductive Biology and Biotechnology, Ministry of Education, Inner Mongolia University, Hohhot, China; Department of Agrobiology, Faculty of Agriculture, Niigata University, Nishi-ku, Niigata, Japan.
The Key Laboratory for Mammalian Reproductive Biology and Biotechnology, Ministry of Education, Inner Mongolia University, Hohhot, China.
Meat Sci. 2015 Oct;108:28-31. doi: 10.1016/j.meatsci.2015.05.017. Epub 2015 May 21.
The septin 7 (CDC10) gene, involved in cellular proliferation, has been previously shown to be expressed at different levels in the longissimus muscle (LM) between low-marbled and high-marbled steer groups by differential-display PCR. It is located within the genomic region of a quantitative trait locus for marbling, and thus was considered as a positional functional candidate gene for marbling. In this study, we showed that the CDC10 expression levels in the LM were positively correlated with marbling in Japanese Black (JB) steers (P<0.0001). Further, an association analysis indicated that a SNP in the promoter region of the CDC10 gene was associated with marbling using 99 JB sires (P=0.03) and 542 JB paternal half-sib progeny steers from a sire homozygous for G allele at the SNP (P<0.0001). These findings suggest possible effects of the expression levels in the LM and the SNP of the CDC10 gene on marbling in JB cattle.
参与细胞增殖的septin 7（CDC10）基因，先前通过差异显示PCR已表明在低大理石花纹和高大理石花纹阉牛组之间的背最长肌（LM）中表达水平不同。它位于大理石花纹数量性状位点的基因组区域内，因此被认为是大理石花纹的位置功能候选基因。在本研究中，我们表明在日本黑牛（JB）阉牛中，LM中的CDC10表达水平与大理石花纹呈正相关（P<0.0001）。此外，关联分析表明，使用99头JB公牛（P=0.03）以及来自该SNP位点G等位基因纯合公牛的542头JB父系半同胞后代阉牛，CDC10基因启动子区域的一个单核苷酸多态性（SNP）与大理石花纹相关（P<0.0001）。这些发现提示了LM中的表达水平和CDC10基因的SNP对JB牛大理石花纹可能产生的影响。
