Oita Prefectural Institute of Animal Industry, Takeda, Oita, Japan.
Anim Sci J. 2010 Feb;81(1):142-4. doi: 10.1111/j.1740-0929.2009.00704.x.
Our previous study detected a single nucleotide polymorphism (SNP), g.1471620G>T, in the 5' flanking region of the endothelial differentiation sphingolipid G-protein-coupled receptor 1 (EDG1) gene, which has been considered as a positional functional candidate for the gene responsible for marbling, and showed association of the g.1471620G>T SNP with marbling in Japanese Black beef cattle. In the present study, we investigated the allele frequency distribution of the g.1471620G>T SNP among the 5 cattle breeds, Japanese Black, Japanese Brown, Japanese Short Horn, Holstein, and Brown Swiss breeds. The T allele at the g.1471620G>T SNP associated with high marbling was found at high frequency in Japanese Black breed that has been subjected to a strong selection for high marbling, while the allele was absent or at very low frequencies in the other breeds that have not been strongly selected for high marbling. Based on this finding, we hypothesized that the pressure of the strong selection for high marbling in Japanese Black breed has increased the frequency of the T allele at the g.1471620G>T SNP in the EDG1.
我们之前的研究检测到内皮分化鞘氨醇 G 蛋白偶联受体 1（EDG1）基因 5'侧翼区的单核苷酸多态性（SNP），g.1471620G>T，该 SNP 被认为是与大理石花纹相关的基因的位置功能候选基因，并且与日本黑牛肉牛的大理石花纹有关。在本研究中，我们调查了 g.1471620G>T SNP 在 5 个牛品种（日本黑牛、日本褐牛、日本短角牛、荷斯坦牛和瑞士褐牛）中的等位基因频率分布。在日本黑牛品种中，与高大理石花纹相关的 g.1471620G>T SNP 的 T 等位基因在经历了强烈选择高大理石花纹的情况下高频出现，而在其他未强烈选择高大理石花纹的品种中，该等位基因缺失或频率非常低。基于这一发现，我们假设日本黑牛品种对高大理石花纹的强烈选择压力增加了 EDG1 中 g.1471620G>T SNP 的 T 等位基因频率。
