Pytlík R, Kideryová L, Benesová K, Cechová H, Veselá R, Rychtrmocová H, Trnený M
Charles University in Prague, First Faculty of Medicine and General University Hospital, First Medical Department-Clinical Department of Haematooncology, U nemocnice 2, Prague, Czech Republic.
Folia Biol (Praha). 2010;56(1):32-5.
We have studied the number of endothelial precursor cells in eighteen patients undergoing allogeneic haematopoietic stem cell transplantation. Endothelial precursor cells were evaluated by colony-forming assay and compared to healthy controls. Patients undergoing allogeneic haematopoietic stem cell transplantation had significantly lower numbers of endothelial precursor cells before the procedure than healthy controls. The numbers of endothelial precursor cells were even lower in the first year after the treatment and seemed to recover partially after twelve months, but even then, they were lower than in healthy volunteers. On the other hand, the number of circulating CD146+CD31+ mature endothelial cells were higher than in healthy controls after more than a one-year follow-up. We hypothesize that lower numbers of endothelial precursor cells and higher numbers of endothelial cells in patients undergoing allogeneic haematopoietic stem cell transplantation reflect ongoing endothelial damage, probably caused by immunological mechanisms, and that this longterm damage may explain the higher risk of cardiovascular events in allogeneic haematopoietic stem cell transplant survivors.
我们研究了18例接受异基因造血干细胞移植患者的内皮祖细胞数量。通过集落形成试验评估内皮祖细胞，并与健康对照进行比较。接受异基因造血干细胞移植的患者在手术前的内皮祖细胞数量明显低于健康对照。治疗后的第一年，内皮祖细胞数量更低，并且在12个月后似乎有部分恢复，但即便如此，其数量仍低于健康志愿者。另一方面，经过一年多的随访，循环中CD146+CD31+成熟内皮细胞的数量高于健康对照。我们推测，接受异基因造血干细胞移植患者的内皮祖细胞数量减少和内皮细胞数量增加反映了持续的内皮损伤，这可能是由免疫机制引起的，并且这种长期损伤可能解释了异基因造血干细胞移植幸存者心血管事件风险较高的原因。
