Chan P J, Tredway D R, Su B C, Corselli J, Davidson B, Ren S
Department of Gynecology and Obstetrics, Loma Linda University Medical Center, California 92350.
J In Vitro Fert Embryo Transf. 1991 Feb;8(1):37-40. doi: 10.1007/BF01131589.
Semen analyses carried out as part of the clinical in vitro fertilization or intrauterine insemination protocols provide important information that determine the type of clinical treatment of the male partner and the sperm processing method. It is postulated that the sperm of male-factor patients cannot survive hypoosmotic stress conditions because of defective sperm membrane function. To test this, 0.1 ml of semen from each of 102 patients was placed in 1.0 ml of 150 mosmol/liter eosin citrate fructose solution and incubated for 30 min at 37 degrees C. The percentage viability of the sperm cells was then determined. The results indicated that patients with two or more abnormal semen parameters had a significantly lower percentage viability while in the hypoosmotic solution (40.6 +/- 4.7%), in contrast to non-male-factor patients (69.0 +/- 1.6%). Donor sperm (N = 32) serving as controls (73.3 +/- 2.1%) had a viability in hypoosmotic solution similar to that of non-male-factor patients. The data suggest that sperm of male-factor patients are less able to survive the hypoosmotic stress conditions as shown by the percentage viability in hypoosmotic solution and emphasize the importance of using less stressful sperm processing methods for in vitro fertilization or insemination in these patients.
作为临床体外受精或宫腔内人工授精方案一部分所进行的精液分析，提供了重要信息，这些信息决定了男性伴侣的临床治疗类型和精子处理方法。据推测，男性因素患者的精子由于精子膜功能缺陷而无法在低渗应激条件下存活。为了验证这一点，将102名患者每人0.1毫升精液置于1.0毫升150毫渗摩尔/升的枸橼酸伊红果糖溶液中，并在37摄氏度下孵育30分钟。然后测定精子细胞的活力百分比。结果表明，与非男性因素患者（69.0±1.6%）相比，有两项或更多精液参数异常的患者在低渗溶液中的活力百分比显著降低（40.6±4.7%）。作为对照的供体精子（N = 32）（73.3±2.1%）在低渗溶液中的活力与非男性因素患者相似。数据表明，男性因素患者的精子在低渗应激条件下存活能力较差，低渗溶液中的活力百分比证明了这一点，并强调了在这些患者的体外受精或授精中使用压力较小的精子处理方法的重要性。
