Daya S, Gunby J, Kohut J
Department of Obstetrics and Gynecology, McMaster University, Hamilton, Ontario, Canada.
Am J Obstet Gynecol. 1989 Nov;161(5):1284-9. doi: 10.1016/0002-9378(89)90684-4.
In vitro fertilization treatment for male infertility is not very successful because fertilization is known to be affected by semen quality. Information on fertilizing ability may provide prognostic information for couples contemplating such treatment. The purpose of this study was to identify semen variables that would predict fertilization and embryo cleavage. Sperm was prepared by the swim-up method before insemination of oocytes obtained by laparoscopy after ovulation induction. Routine semen analysis and the hypoosmotic swelling test for assessment of sperm membrane integrity were performed on aliquots of prepared sperm. Logistic regression and receiver-operator characteristic curve analyses were performed to determine the overall best-fitting model and discriminatory level of variables that would predict cleavage. The results indicate that after the swim-up procedure, at least 10 million sperm/ml, capable of undergoing swelling in hypoosmotic medium, are necessary to increase the likelihood of in vitro fertilization and cleavage.
体外受精治疗男性不育症的成功率不高，因为已知受精会受到精液质量的影响。关于受精能力的信息可能为考虑这种治疗的夫妇提供预后信息。本研究的目的是确定能够预测受精和胚胎分裂的精液变量。在通过排卵诱导后经腹腔镜获得的卵母细胞授精前，采用上游法制备精子。对制备好的精子 aliquots 进行常规精液分析和用于评估精子膜完整性的低渗肿胀试验。进行逻辑回归和受试者工作特征曲线分析，以确定能够预测分裂的总体最佳拟合模型和变量的鉴别水平。结果表明，在上游法处理后，至少每毫升 1000 万个能够在低渗培养基中发生肿胀的精子，对于提高体外受精和分裂的可能性是必要的。
