Jones M C, Gough K, Dasgupta I, Rao B L, Cliffe J, Qu R, Shen P, Kaniewska M, Blakebrough M, Davies J W
John Innes Institute, AFRC Institute for Plant Science Research, Norwich, U.K.
J Gen Virol. 1991 Apr;72 ( Pt 4):757-61. doi: 10.1099/0022-1317-72-4-757.
We present evidence that rice tungro spherical virus (RTSV) has a genome of polyadenylated single-stranded RNA of about 10 kb whereas rice tungro bacilliform virus (RTBV) contains double-stranded circular DNA. RTBV DNA has been mapped and shown to have two discontinuities, one in each strand, at specific sites; it thus resembles that of the caulimoviruses. Gel electrophoresis of RTSV preparations revealed two protein bands (Mr 35K and 26K). RTBV yielded two major protein bands of 37K and 33K together with several minor species of higher and lower Mr which react with antiviral serum.
我们提供的证据表明,水稻东格鲁球形病毒(RTSV)具有约10 kb的多聚腺苷酸化单链RNA基因组,而水稻东格鲁杆状病毒(RTBV)则含有双链环状DNA。RTBV DNA已被定位,并显示在特定位点的每条链上都有两个不连续处;因此它类似于花椰菜花叶病毒的DNA。RTSV制剂的凝胶电泳显示出两条蛋白带(分子量分别为35K和26K)。RTBV产生了两条主要的蛋白带,分子量分别为37K和33K,以及几种分子量较高和较低的次要蛋白条带,这些条带能与抗病毒血清发生反应。