Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi 110 021, India.
J Biosci. 2020;45.
Rice tungro is a serious viral disease of rice resulting from infection by two viruses, Rice tungro bacilliform virus and Rice tungro spherical virus. To gain molecular insights into the global gene expression changes in rice during tungro, a comparative whole genome transcriptome study was performed on healthy and tungroaffected rice plants using Illumina Hiseq 2500. About 10 GB of sequenced data comprising about 50 million paired end reads per sample were then aligned on to the rice genome. Gene expression analysis revealed around 959 transcripts, related to various cellular pathways concerning stress response and hormonal homeostasis to be differentially expressed. The data was validated through qRT-PCR. Gene ontology and pathway analyses revealed enrichment of transcripts and processes similar to the differentially expressed genes categories. In short, the present study is a comprehensive coverage of the differential gene expression landscape and provides molecular insights into the infection dynamics of the rice-tungro virus system.
水稻黑条矮缩病是一种由两种病毒引起的严重水稻病毒性疾病，即水稻黑条矮缩病毒和水稻曲叶病毒。为了深入了解水稻感染黑条矮缩病后的全球基因表达变化，我们使用 Illumina Hiseq 2500 对健康和感染黑条矮缩病的水稻植株进行了全基因组转录组比较研究。每个样本的测序数据约为 10GB，包含约 5000 万个配对末端reads，然后将其映射到水稻基因组上。基因表达分析显示，约有 959 个与应激反应和激素动态平衡等各种细胞途径相关的转录本存在差异表达。通过 qRT-PCR 对数据进行了验证。基因本体论和途径分析显示，转录本和过程的富集与差异表达基因类别相似。总之，本研究全面涵盖了差异基因表达图谱，并为水稻-黑条矮缩病毒系统的感染动态提供了分子见解。
