Department of Pathology, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China.
J Med Virol. 2010 Apr;82(4):658-67. doi: 10.1002/jmv.21731.
To investigate the clinicopathologic features, Epstein-Barr virus (EBV) latency pattern and genome polymorphism of EBV-associated gastric carcinoma (EBVaGC) in Guangzhou, an endemic area of nasopharyngeal carcinoma (NPC), an in situ hybridization assay of EBV-encoded small RNA-1 (EBER-1) was used to identify the presence of EBV in 676 consecutive gastric carcinoma cases. EBV-encoded proteins EBNA1, EBNA2, LMP1, and ZEBRA were detected by immunohistochemistry. EBV genome polymorphism was also analyzed by PCR and DNA sequencing. Of the 676 cases, 45 EBV-positive cases (6.7%) were identified, including 37 (8.5%) male and 8 (3.3%) female cases. EBNA1 was detected in 42 cases (93.3%), while EBNA2, LMP1, and ZEBRA were all negative. In the EBV genome polymorphism analysis, type A strain, prototype F, type I, XhoI-, and del-LMP1 variants were predominant among EBVaGC patients, accounting for 44 (97.8%), 37 (82.2%), 45 (100%), 34 (75.6%), and 42 (93.3%) cases, respectively. Moreover, a new hotspot mutation in the BamHI-W1/I1 boundary region (148,972 T --> C) was found in 39 (86.7%) of the 45 cases. The predominant EBV variants in EBVaGC in Guangzhou are prototype F, type I, and XhoI-, which are different from those in NPC in this area (predominant variant-type "f") and in EBVaGC in Latin American countries (predominant type "i" and XhoI+), suggesting that the EBV variants are not only geographically distributed but also disease restricted, and the pathogenic role of EBV in different EBV associated epithelial malignancies in different areas may be distinct.
为了研究在鼻咽癌高发地区广州的胃腺癌（EBVaGC）的临床病理特征、EBV 潜伏模式和基因组多态性，我们使用原位杂交法检测了 676 例连续胃癌病例中 EBV 编码的小 RNA-1（EBER-1）的存在，以确定 EBV 的存在。通过免疫组织化学检测 EBV 编码蛋白 EBNA1、EBNA2、LMP1 和 ZEBRA。还通过 PCR 和 DNA 测序分析了 EBV 基因组多态性。在 676 例病例中，发现 45 例 EBV 阳性病例（6.7%），包括 37 例男性（8.5%）和 8 例女性（3.3%）。EBNA1 在 42 例病例（93.3%）中检测到，而 EBNA2、LMP1 和 ZEBRA 均为阴性。在 EBV 基因组多态性分析中，A型株、原型 F、I 型、XhoI-和 del-LMP1 变异株在 EBVaGC 患者中占优势，分别占 44 例（97.8%）、37 例（82.2%）、45 例（100%）、34 例（75.6%）和 42 例（93.3%）。此外，在 45 例中的 39 例（86.7%）中发现了 BamHI-W1/I1 边界区域的新热点突变（148,972 T --> C）。在广州的 EBVaGC 中，主要的 EBV 变异型是原型 F、I 型和 XhoI-，与该地区的 NPC（主要变异型“f”）和拉丁美洲国家的 EBVaGC（主要类型“i”和 XhoI+）不同，提示 EBV 变异型不仅具有地理分布性，而且具有疾病局限性，不同地区不同 EBV 相关上皮性恶性肿瘤中 EBV 的致病作用可能不同。
