Muldoon Mark T, Li Jingkun, Sutzko Meredith, Olsson-Allen Ann Christine, Teaney George, Gonzalez Verapaz
Strategic Diagnostics Inc., 128 Sandy Dr, Newark, DE 19713, USA.
J AOAC Int. 2009 Nov-Dec;92(6):1890-4.
RapidChek SELECT Salmonella was previously validated in the Performance Tested Methods program for the detection of Salmonella spp. in raw ground chicken, chicken carcass rinse, sliced cooked turkey, and liquid eggs. The present matrix extension study conducted under the AOAC Research Institute Emergency Response Validation program compared the RapidChek SELECT Salmonella method to the U.S. Food and Drug Administration's Bacteriological Analytical Manual (FDA-BAM) method for the detection of Salmonella Typhimurium in peanut butter. Overall, 27 samples were found positive by the RapidChek SELECT Salmonella method and 27 were found to be positive by the reference method. All RapidChek SELECT Salmonella presumptive positives were confirmed positive by the cultural reference method; additionally, all presumptive negative results were confirmed negative by the cultural reference method. Accordingly 0% false-negative rate and 0% false-positive rate were found. No significant difference between the RapidCheck SELECT Salmonella and FDA-BAM reference method was found; calculated Chi-square was 0. Results indicate that a low level of Salmonella in peanut butter can be successfully recovered and detected in the minimum 24 h enrichment protocol.
RapidChek SELECT沙门氏菌检测方法先前已在性能验证方法项目中得到验证，可用于检测生碎鸡肉、鸡胴体冲洗液、切片熟火鸡肉和液态蛋中的沙门氏菌属。在AOAC研究所应急响应验证项目下进行的本次基质扩展研究，将RapidChek SELECT沙门氏菌检测方法与美国食品药品监督管理局的《细菌学分析手册》（FDA - BAM）方法用于检测花生酱中鼠伤寒沙门氏菌进行了比较。总体而言，RapidChek SELECT沙门氏菌检测方法检测出27个阳性样本，参考方法检测出27个阳性样本。所有RapidChek SELECT沙门氏菌初步阳性结果均经培养参考方法确认为阳性；此外，所有初步阴性结果也经培养参考方法确认为阴性。因此，假阴性率和假阳性率均为0%。未发现RapidCheck SELECT沙门氏菌检测方法与FDA - BAM参考方法之间存在显著差异；计算得到的卡方值为0。结果表明，在最短24小时富集方案中，花生酱中低水平的沙门氏菌能够成功回收并检测出来。
