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食品防护沙门氏菌检测试剂盒。性能测试方法120301。

Foodproof Salmonella Detection Kit. Performance Tested Method 120301.

作者信息

Lindhardt Charlotte, Schönenbrücher Holger, Slaghuis Jörg, Bubert Andreas, Ossmer Rolf, Junge Benjamin, Berghof-Jäger Kornelia

机构信息

Merck KGaA, Frankfurter Strasse 250, 64293 Darmstadt, Germany.

出版信息

J AOAC Int. 2009 Nov-Dec;92(6):1876-84.

Abstract

The foodproof Salmonella Detection Kit was previously validated in the Performance Tested Methods program for the detection of Salmonella species in a variety of foods, including milk powder, egg powder, coconut, cocoa powder, chicken breast, minced meat, sliced sausage, sausage, smoked fish, pasta, white pepper, cumin, dough, wet pet food, dry pet food, ice cream, watermelon, sliced cabbage, food dye, and milk chocolate. The method was shown to be equivalent to the U.S. Food and Drug Administration's Bacteriological Analytical Manual (FDA-BAM) and the U.S. Department of Agriculture, Food Safety and Inspection Service's Microbiology Laboratory Guidebook reference culture procedures. In the first Emergency Response Validation (ERV) extension study, peanut butter was inoculated with S. enterica. ser Typhimurium. For the low inoculation level (1.08 CFU/25 g), a Chi-square value of 2.25 indicated that there was no significant performance difference between the foodproof Salmonella Detection Kit and the FDA-BAM reference method. For high-level inoculation (11.5 CFU/25 g) and uninoculated control, there was 100% agreement between the methods. In the second ERV extension study, peanut butter was inoculated with S. enterica. ser Typhimurium. For both inoculation levels (0.1 and 0.5 CFU/25 g by most probable number), Chi-square values of 0 indicated that there was no significant performance difference between foodproof Salmonella Detection Kit and the FDA-BAM reference method.

摘要

耐食品污染沙门氏菌检测试剂盒先前在性能验证方法项目中得到验证,可用于检测多种食品中的沙门氏菌,包括奶粉、蛋粉、椰子、可可粉、鸡胸肉、碎肉、切片香肠、香肠、烟熏鱼、意大利面、白胡椒、孜然、面团、湿宠物食品、干宠物食品、冰淇淋、西瓜、切片卷心菜、食用色素和牛奶巧克力。该方法被证明等同于美国食品药品监督管理局的《细菌学分析手册》(FDA - BAM)以及美国农业部食品安全与检验局的《微生物学实验室指南》参考培养程序。在首次应急响应验证(ERV)扩展研究中,用肠炎沙门氏菌鼠伤寒血清型接种花生酱。对于低接种水平(1.08 CFU/25 g),卡方值为2.25,表明耐食品污染沙门氏菌检测试剂盒与FDA - BAM参考方法之间在性能上无显著差异。对于高接种水平(11.5 CFU/25 g)和未接种对照,两种方法的一致性为100%。在第二次ERV扩展研究中,用肠炎沙门氏菌鼠伤寒血清型接种花生酱。对于两种接种水平(最可能数法为0.1和0.5 CFU/25 g),卡方值为0,表明耐食品污染沙门氏菌检测试剂盒与FDA - BAM参考方法之间在性能上无显著差异。

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