Lindhardt Charlotte, Schönenbrücher Holger, Slaghuis Jörg, Bubert Andreas, Ossmer Rolf
Merck KGaA, Frankfurter Strasse 250, 64293 Darmstadt, Germany.
J AOAC Int. 2009 Nov-Dec;92(6):1885-9.
Singlepath Salmonella is an immunochromatographic (lateral flow) assay for the presumptive qualitative detection of Salmonella spp. in food. A previous AOAC Performance Tested Method study evaluated Singlepath Salmonella as an effective method for the detection of Salmonella spp. in the following selected foods: dried skimmed milk, black pepper, dried pet food, desiccated coconut, cooked peeled frozen prawns, raw ground beef, and raw ground turkey. In this Emergency Response Validation extension, creamy peanut butter was inoculated with S. enterica. ser. Typhimurium. For low contamination level (1.08 CFU/25 g), a Chi-square value of 0.5 indicated that there was no significant difference between Singlepath Salmonella and the U.S. Food and Drug Administration's Bacteriological Analytical Manual (FDA-BAM) reference method. For high-level and uninoculated control there was 100% agreement between the methods.
单通道沙门氏菌检测是一种用于食品中沙门氏菌属定性推定检测的免疫层析（侧向流动）分析方法。美国分析化学家协会（AOAC）先前的性能验证方法研究评估了单通道沙门氏菌检测作为在以下选定食品中检测沙门氏菌属的有效方法：脱脂奶粉、黑胡椒、干宠物食品、椰丝、熟冻去壳虾、生牛肉末和生火鸡肉末。在本次应急响应验证扩展中，肠炎沙门氏菌血清型鼠伤寒沙门氏菌接种于奶油花生酱中。对于低污染水平（1.08 CFU/25 g），卡方值为0.5表明单通道沙门氏菌检测与美国食品药品监督管理局的《细菌学分析手册》（FDA - BAM）参考方法之间没有显著差异。对于高污染水平样本和未接种对照样本，两种方法的结果完全一致。
