Centre for Blood Research and the Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z3.
J Am Chem Soc. 2010 Mar 17;132(10):3423-30. doi: 10.1021/ja909174x.
Macromolecular cell surface modification techniques have shown tremendous utility in various biomedical applications. However, a major drawback concerns inefficient cell surface modification caused by the poor association of hydrophilic macromolecules with cell surfaces. Here, a novel, highly efficient, and universal strategy in which nonreactive "additive" macromolecules are used to modulate the grafting efficiency of cell surface reactive, hydrophilic macromolecules is described. Unprecedented enhanced cell surface modifications by up to 10-fold were observed when various concentrations of a suitable "additive" polymer was present with a constant and low concentration of a "reactive" macromolecule. The importance of this increased efficiency and the possible mechanisms involved are discussed. The cell compatible technique is demonstrated in the case of four different cell types--red blood cells (RBC), leukocytes, platelets, and Jurkat cells. A practical application of grafting macromolecules to cell surfaces in concentrated polymer solutions is demonstrated by the enhanced camouflage of RBC surface antigens for the development of RhD null RBC. In principle, the technique can be adapted to various macromolecular systems and cell types, with significant potential for biomedical applications such as live cell based technologies.
高分子细胞表面修饰技术在各种生物医学应用中显示出巨大的效用。然而，一个主要的缺点是由于亲水分子与细胞表面的结合不良，导致细胞表面修饰效率低下。在这里，我们描述了一种新颖的、高效的和通用的策略，其中非反应性的“添加剂”高分子可以调节细胞表面反应性亲水分子的接枝效率。当存在各种浓度的合适的“添加剂”聚合物时，观察到各种反应性大分子的接枝效率提高了 10 倍。讨论了这种效率提高的重要性和可能涉及的机制。该细胞兼容技术在四种不同的细胞类型——红细胞 (RBC)、白细胞、血小板和 Jurkat 细胞中得到了验证。通过增强 RhD 阴性 RBC 表面抗原的伪装，证明了在高浓度聚合物溶液中接枝大分子到细胞表面的实际应用。原则上，该技术可以适应各种高分子系统和细胞类型，在基于活细胞的技术等生物医学应用中有很大的潜力。
