Nadji Seyed Alireza, Poos-Ashkan Leila, Khalilzadeh Soheila, Baghaie Nooshin, Shiraghaei Mohammad Jafar, Hassanzad Maryam, Bolursaz Mohammad Reza
Virology Research Centre, National Research Institute for Tuberculosis and Lung Disease NRITLD, Masih Daneshvari Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Scand J Infect Dis. 2010 Aug;42(8):598-603. doi: 10.3109/00365540903582442.
Human bocavirus (hBoV) was first discovered in respiratory samples from children in 2005, and has been classified in the Parvoviridae family. hBoV has also been detected in children with acute gastroenteritis. This study was performed to analyze the frequency and phylogeny of hBoV in the respiratory and stool samples of children with acute respiratory tract illnesses and gastroenteritis during the time period beginning 2006 and ending 2008, at the Virology Research Centre, Masih Daneshvari Hospital, NRITLD, Tehran, Iran. Respiratory and stool samples were screened for hBoV by nested polymerase chain reaction with primers from the NS-1 gene. Nine out of 133 respiratory samples (6.8%) and 6 out of 47 stool samples (12.8%) were positive for hBoV. Ten positive samples (7 respiratory and 3 stool samples) were subjected to phylogenetic analysis by sequencing a fragment of the VP1/VP2 gene junction. The results showed a high similarity among isolates (>or=99%). It was found that hBoV isolates can be divided into 3 genetic groups.
人博卡病毒（hBoV）于2005年首次在儿童呼吸道样本中被发现，并已被归类于细小病毒科。在患有急性肠胃炎的儿童中也检测到了hBoV。本研究旨在分析2006年初至2008年末期间，在伊朗德黑兰马西赫·达内什瓦里医院病毒学研究中心，患有急性呼吸道疾病和肠胃炎的儿童呼吸道和粪便样本中hBoV的检出频率及系统发育情况。采用针对NS-1基因的引物，通过巢式聚合酶链反应对呼吸道和粪便样本进行hBoV筛查。133份呼吸道样本中有9份（6.8%）hBoV呈阳性，47份粪便样本中有6份（12.8%）hBoV呈阳性。对10份阳性样本（7份呼吸道样本和3份粪便样本）通过对VP1/VP2基因连接处的片段进行测序进行系统发育分析。结果显示分离株之间具有高度相似性（≥99%）。研究发现hBoV分离株可分为3个基因群。
