喀麦隆雅温得急性呼吸道感染儿童中检测到的人博卡病毒的系统发育分析。
Phylogenic analysis of human bocavirus detected in children with acute respiratory infection in Yaounde, Cameroon.
作者信息
Kenmoe Sebastien, Vernet Marie-Astrid, Njankouo-Ripa Mohamadou, Penlap Véronique Beng, Vabret Astrid, Njouom Richard
机构信息
Virology Unit, Centre Pasteur of Cameroon, Yaounde, BP 1274, Yaounde, Cameroon.
Biochemistry Department, Université of Yaounde 1, Yaounde, Cameroon.
出版信息
BMC Res Notes. 2017 Jul 17;10(1):293. doi: 10.1186/s13104-017-2620-y.
OBJECTIVE
Human Bocavirus (HBoV) was first identified in 2005 and has been shown to be a common cause of respiratory infections and gastroenteritis in children. In a recent study, we found that 10.7% of children with acute respiratory infections (ARI) were infected by HBoV. Genetic characterization of this virus remains unknown in Central Africa, particularly in Cameroon Leeding us to evaluate the molecular characteristics of HBoV strains in Cameroonian children with ARI.
RESULTS
Phylogenetic analysis of partial HBoV VP1/2 sequences showed a low level of nucleotide variation and the circulation of HBoV genotype 1 (HBoV-1) only. Three clades were obtained, two clustering with each of the reference strains ST1 and ST2, and a third group consisting of only Cameroon strains. By comparing with the Swedish reference sequences, ST1 and ST2, Cameroon sequences showed nucleotide and amino acid similarities of respectively 97.36-100% and 98.35-100%. These results could help improve strategies for monitoring and control of respiratory infections in Cameroon.
目的
人博卡病毒（HBoV）于2005年首次被发现，已被证明是儿童呼吸道感染和胃肠炎的常见病因。在最近的一项研究中，我们发现10.7%的急性呼吸道感染（ARI）儿童感染了HBoV。在中非，特别是在喀麦隆，这种病毒的基因特征仍然未知，这促使我们评估喀麦隆ARI儿童中HBoV毒株的分子特征。
结果
对部分HBoV VP1/2序列的系统发育分析显示核苷酸变异水平较低，且仅存在HBoV基因型1（HBoV-1）的传播。获得了三个进化枝，其中两个分别与参考菌株ST1和ST2聚类，第三个组仅由喀麦隆菌株组成。通过与瑞典参考序列ST1和ST2比较，喀麦隆序列的核苷酸和氨基酸相似性分别为97.36 - 100%和98.35 - 100%。这些结果有助于改进喀麦隆呼吸道感染的监测和控制策略。
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