Department of Biology, University of Rochester, Rochester, NY 14627, USA.
Insect Mol Biol. 2010 Feb;19 Suppl 1(Suppl 1):99-107. doi: 10.1111/j.1365-2583.2009.00934.x.
We report three novel small RNA viruses uncovered from cDNA libraries from parasitoid wasps in the genus Nasonia. The genome of this kind of virus is a positive-sense single-stranded RNA with a 3' poly(A), which facilitates cloning from cDNAs. Two of the viruses, NvitV-1 and NvitV-2, possess a RNA-dependent RNA polymerase that associates them with the family Iflaviridae of the order Picornavirales. A third virus, NvitV-3, is most similar to the Nora virus from Drosophila. A reverse transcription-PCR method developed for NvitV-1 indicates that it is a persistent commensal infection of Nasonia.
我们从寄生蜂大蜡螟属的 cDNA 文库中发现了三种新型的小 RNA 病毒。这类病毒的基因组是一条正链单链 RNA,带有 3' 多聚腺苷酸,便于从 cDNA 中克隆。其中两种病毒,NvitV-1 和 NvitV-2,具有 RNA 依赖性 RNA 聚合酶,使其与小 RNA 病毒目正粘病毒科的 Iflaviridae 家族相关联。第三种病毒 NvitV-3 与果蝇中的 Nora 病毒最为相似。针对 NvitV-1 开发的反转录 PCR 方法表明,它是大蜡螟的一种持续性共生感染。