Division of Clinical Dermatology, Department of Clinical Veterinary Medicine, Vetsuisse Faculty, University of Bern, Switzerland.
Vet Res. 2010 Jul-Aug;41(4):40. doi: 10.1051/vetres/2010012. Epub 2010 Feb 22.
Dendritic cells (DC) represent a heterogeneous cell family of major importance for innate immune responses against pathogens and antigen presentation during infection, cancer, allergy and autoimmunity. The aim of the present study was to characterize canine DC generated in vitro with respect to their phenotype, responsiveness to toll-like receptor (TLR) ligands and T-cell stimulatory capacity. DC were derived from monocytes (MoDC) and from bone marrow hematopoietic cells cultured with either Flt3-ligand (FL-BMDC) or with GM-CSF (GM-BMDC). All three methods generated cells with typical DC morphology that expressed CD1c, CD11c and CD14, similar to macrophages. However, CD40 was only found on DC, CD206 on MPhi and BMDC, but not on monocytes and MoDC. CD1c was not found on monocytes but on all in vitro differentiated cells. FL-BMDC and GM-BMDC were partially positive for CD4 and CD8. CD45RA was expressed on a subset of FL-BMDC but not on MoDC and GM-BMDC. MoDC and FL-DC responded well to TLR ligands including poly-IC (TLR2), Pam3Cys (TLR3), LPS (TLR4) and imiquimod (TLR7) by up-regulating MHC II and CD86. The generated DC and MPhi showed a stimulatory capacity for lymphocytes, which increased upon maturation with LPS. Taken together, our results are the basis for further characterization of canine DC subsets with respect to their role in inflammation and immune responses.
树突状细胞 (DC) 是先天免疫反应中对抗病原体和感染期间抗原呈递的重要异质性细胞家族。本研究旨在对犬体外生成的 DC 的表型、对 Toll 样受体 (TLR) 配体的反应性和 T 细胞刺激能力进行特征分析。从单核细胞 (MoDC) 和骨髓造血细胞中，用 Flt3 配体 (FL-BMDC) 或 GM-CSF (GM-BMDC) 培养生成 DC。所有三种方法均生成具有典型 DC 形态的细胞，表达 CD1c、CD11c 和 CD14，与巨噬细胞相似。然而，CD40 仅在 DC 上表达，CD206 在 MPhi 和 BMDC 上表达，但在单核细胞和 MoDC 上不表达。CD1c 不在单核细胞上表达，但在所有体外分化的细胞上表达。FL-BMDC 和 GM-BMDC 部分呈 CD4 和 CD8 阳性。FL-BMDC 上表达 CD45RA，但 MoDC 和 GM-BMDC 上不表达。MoDC 和 FL-DC 对 TLR 配体（包括 poly-IC（TLR2）、Pam3Cys（TLR3）、LPS（TLR4）和 imiquimod（TLR7））反应良好，通过上调 MHC II 和 CD86 来实现。生成的 DC 和 MPhi 对淋巴细胞具有刺激能力，用 LPS 成熟后这种能力会增强。总之，我们的结果为进一步研究犬 DC 亚群在炎症和免疫反应中的作用奠定了基础。
