State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, People's Republic of China.
Acta Biomater. 2010 Aug;6(8):3013-20. doi: 10.1016/j.actbio.2010.02.015. Epub 2010 Feb 16.
Electrospinning has been recognized as an efficient technique for fabricating polymer nanofibrous biomaterials. However, the study of electrospun inorganic biomaterials with well-designed three-dimensional (3-D) structures is still limited and little reported. In this study hydroxyapatite (HAp) nanorods with an average diameter of approximately 7 nm and length of approximately 27 nm were synthesized through a simple precipitation method and used for the fabrication of inorganic/organic [poly(vinyl pyrolidone) (PVP)] composite nanofibers by electrospinning in ethanol solution. 3-D fabrics and aligned nanofiber arrays of the HAp nanorods/PVP composite were obtained as precursors. Thereafter, 3-D single phase HAp fabrics, tubular structures and aligned nanofiber arrays were obtained after thermal treatment of the corresponding composite precursors. Cytotoxicity experiments indicated that the HAp fabric scaffold had good biocompatibility. In vitro experiments showed that mesenchymal stem cells could attach to the HAp fabric scaffold after culture for 24h.
静电纺丝已被公认为制备聚合物纳米纤维生物材料的有效技术。然而，具有良好设计的三维（3-D）结构的静电纺无机生物材料的研究仍然有限，报道很少。在这项研究中，通过简单的沉淀法合成了平均直径约为 7nm、长度约为 27nm 的羟基磷灰石（HAp）纳米棒，并将其用于通过在乙醇溶液中静电纺丝制备无机/有机[聚乙烯吡咯烷酮（PVP）]复合纳米纤维。作为前体，获得了 3-D 织物和 HAp 纳米棒/PVP 复合的取向纳米纤维阵列。此后，通过相应复合前体的热处理，获得了 3-D 单相 HAp 织物、管状结构和取向纳米纤维阵列。细胞毒性实验表明，HAp 织物支架具有良好的生物相容性。体外实验表明，间充质干细胞在培养 24 小时后可以附着在 HAp 织物支架上。
