College of Textiles, Key Laboratory of Textile Science & Technology, Ministry of Education, Donghua University, Shanghai 201620, China.
Colloids Surf B Biointerfaces. 2010 Sep 1;79(2):315-25. doi: 10.1016/j.colsurfb.2010.03.043. Epub 2010 Apr 3.
Collagen functionalized thermoplastic polyurethane nanofibers (TPU/collagen) were successfully produced by coaxial electrospinning technique with a goal to develop biomedical scaffold. A series of tests were conducted to characterize the compound nanofiber and its membrane in this study. Surface morphology and interior structure of the ultrafine fibers were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM), whereas the fiber diameter distribution was also measured. The crosslinked membranes were also characterized by SEM. Porosities of different kinds of electrospun mats were determined. The surface chemistry and chemical composition of collagen/TPU coaxial nanofibrous membranes were verified by X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectrometry (FTIR). Mechanical measurements were carried out by applying tensile test loads to samples which were prepared from electrospun ultra fine non-woven fiber mats. The coaxial electrospun nanofibers were further investigated as a promising scaffold for PIECs culture. The results demonstrated that coaxial electrospun composite nanofibers had the characters of native extracellular matrix and may be used effectively as an alternative material for tissue engineering and functional biomaterials.
胶原功能化热塑性聚氨酯纳米纤维（TPU/胶原）通过同轴静电纺丝技术成功制备，目的是开发生物医学支架。本研究对复合纳米纤维及其膜进行了一系列测试来进行表征。通过扫描电子显微镜（SEM）、透射电子显微镜（TEM）和原子力显微镜（AFM）对超细微纤维的表面形态和内部结构进行了表征，同时还测量了纤维直径分布。交联膜也通过 SEM 进行了表征。不同类型的静电纺丝垫的孔隙率也进行了测定。通过 X 射线光电子能谱（XPS）和傅里叶变换红外光谱（FTIR）验证了胶原/TPU 同轴纳米纤维膜的表面化学和化学成分。通过对静电纺超细微无纺织物垫制备的样品施加拉伸试验载荷，进行了力学测量。进一步研究了同轴静电纺纳米纤维作为 PIECs 培养的一种有前途的支架。结果表明，同轴静电纺复合纳米纤维具有天然细胞外基质的特性，可用作组织工程和功能生物材料的替代材料。
