Unidad de Proteómica, Universidad Complutense de Madrid-Parque Científico de Madrid (UCM-PCM), Spain.
J Proteomics. 2010 May 7;73(7):1404-9. doi: 10.1016/j.jprot.2010.02.008. Epub 2010 Feb 16.
We have set up a fast and easy methodology to identify cell-surface proteins in live yeasts. A non-gel proteomic approach was based on a short period of trypsin treatment followed by peptide separation and identification using nano-LC followed by off-line MS/MS. Candida albicans was used as a model organism and proteins involved in cell wall organization, cell rescue, defense, virulence, transport, protein fate and metabolism were identified. This strategy is a powerful tool to study host-pathogen interactions and to look for potential vaccine candidates and drug targets.
我们建立了一种快速而简单的方法来鉴定活酵母中的细胞表面蛋白。一种非凝胶蛋白质组学方法基于短时间的胰蛋白酶处理，然后使用纳升液相色谱和离线 MS/MS 进行肽分离和鉴定。我们选择白色念珠菌作为模型生物，鉴定了参与细胞壁组织、细胞拯救、防御、毒力、运输、蛋白质命运和代谢的蛋白质。这种策略是研究宿主-病原体相互作用以及寻找潜在疫苗候选物和药物靶点的有力工具。
