Kubota S, Nosaka T, Cullen B R, Maki M, Hatanaka M
Institute for Virus Research, Kyoto University, Japan.
J Virol. 1991 May;65(5):2452-6. doi: 10.1128/JVI.65.5.2452-2456.1991.
Two chimeric mutant genes derived from rev of human immunodeficiency virus type 1 and rex of human T-cell leukemia virus type I were constructed to investigate the functions of the nucleolar-targeting signals (NOS) in Rev and Rex proteins. A chimeric Rex protein whose NOS region was substituted with the NOS of Rev was located predominantly in the cell nucleolus and functioned like the wild-type protein in the Rex assay system. However, a chimeric Rev with the NOS of Rex abolished Rev function despite its nucleolar localization. This nonfunctional nucleolar-targeting chimeric protein inhibited the function of both Rex and Rev. In the same experimental conditions, this mutant interfered with the localization of the functional Rex in the nucleolus.
构建了两个嵌合突变基因，它们分别来源于1型人类免疫缺陷病毒的Rev和1型人类T细胞白血病病毒的Rex，以研究Rev和Rex蛋白中核仁靶向信号（NOS）的功能。一种嵌合Rex蛋白，其NOS区域被Rev的NOS取代，主要定位于细胞核仁，并且在Rex检测系统中其功能与野生型蛋白相似。然而，具有Rex的NOS的嵌合Rev尽管定位于核仁，但却丧失了Rev功能。这种无功能的核仁靶向嵌合蛋白抑制了Rex和Rev的功能。在相同的实验条件下，该突变体干扰了功能性Rex在核仁中的定位。
