Instituto Gulbenkian de Ciência, Oeiras, Portugal.
EMBO Rep. 2010 Mar;11(3):194-200. doi: 10.1038/embor.2010.5. Epub 2010 Feb 19.
In animal cells the centrosome is positioned at the cell centre in close association with the nucleus. The mechanisms responsible for this are not completely understood. Here, we report the first characterization of human TBCC-domain containing 1 (TBCCD1), a protein related to tubulin cofactor C. TBCCD1 localizes at the centrosome and at the spindle midzone, midbody and basal bodies of primary and motile cilia. Knockdown of TBCCD1 in RPE-1 cells caused the dissociation of the centrosome from the nucleus and disorganization of the Golgi apparatus. TBCCD1-depleted cells are larger, less efficient in primary cilia assembly and their migration is slower in wound-healing assays. However, the major microtubule-nucleating activity of the centrosome is not affected by TBCCD1 silencing. We propose that TBCCD1 is a key regulator of centrosome positioning and consequently of internal cell organization.
在动物细胞中，中心体位于细胞中心，与细胞核密切相关。负责这一过程的机制尚不完全清楚。在这里，我们首次描述了人类 TBCC 结构域包含蛋白 1（TBCCD1），一种与微管共因子 C 相关的蛋白质。TBCCD1 定位于中心体和纺锤体中部、原纤毛和运动纤毛的中体和基体。在 RPE-1 细胞中敲低 TBCCD1 会导致中心体与细胞核分离以及高尔基体的紊乱。TBCCD1 耗尽的细胞更大，初级纤毛组装效率较低，在划痕愈合实验中迁移速度较慢。然而，中心体的主要微管成核活性不受 TBCCD1 沉默的影响。我们提出 TBCCD1 是中心体定位的关键调节因子，因此也是细胞内部组织的关键调节因子。
