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非浸润性脑膜瘤中波形蛋白磷酸化增加。

Increased phosphorylation of vimentin in noninfiltrative meningiomas.

机构信息

Grenoble Institut des Neurosciences, INSERM U836, Université Joseph Fourier, Grenoble, France.

出版信息

PLoS One. 2010 Feb 16;5(2):e9238. doi: 10.1371/journal.pone.0009238.

DOI:10.1371/journal.pone.0009238
PMID:20169076
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2821924/
Abstract

BACKGROUND

Tissue invasion or tissue infiltration are clinical behaviors of a poor-prognosis subset of meningiomas. We carried out proteomic analyses of tissue extracts to discover new markers to accurately distinguish between infiltrative and noninfiltrative meningiomas.

METHODOLOGY/PRINCIPAL FINDINGS: Protein lysates of 64 different tissue samples (including two brain-invasive and 32 infiltrative tumors) were submitted to SELDI-TOF mass spectrometric analysis. Mass profiles were used to build up both unsupervised and supervised hierarchical clustering. One marker was found at high levels in noninvasive and noninfiltrative tumors and appeared to be a discriminative marker for clustering infiltrative and/or invasive meningiomas versus noninvasive meningiomas in two distinct subsets. Sensitivity and specificity were 86.7% and 100%, respectively. This marker was purified and identified as a multiphosphorylated form of vimentin, a cytoskeletal protein expressed in meningiomas.

CONCLUSIONS/SIGNIFICANCE: Specific forms of vimentin can be surrogate molecular indicators of the invasive/infiltrative phenotype in tumors.

摘要

背景

组织侵犯或组织浸润是脑膜瘤预后不良亚群的临床行为。我们进行了组织提取物的蛋白质组学分析，以发现新的标记物来准确区分浸润性和非浸润性脑膜瘤。

方法/主要发现：对 64 个不同组织样本（包括 2 个脑侵袭性和 32 个浸润性肿瘤）的蛋白裂解物进行了 SELDI-TOF 质谱分析。质量谱用于建立无监督和有监督的层次聚类。在非侵袭性和非浸润性肿瘤中发现了一个高水平的标记物，它似乎是一个有区别性的标记物，用于将浸润性和/或侵袭性脑膜瘤与非侵袭性脑膜瘤聚类在两个不同的亚集中。敏感性和特异性分别为 86.7%和 100%。该标记物被纯化并鉴定为波形蛋白的多磷酸化形式，一种在脑膜瘤中表达的细胞骨架蛋白。

结论/意义：波形蛋白的特定形式可以作为肿瘤侵袭/浸润表型的替代分子指标。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c59/2821924/3d3544606a06/pone.0009238.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c59/2821924/c1d3d65a1a21/pone.0009238.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c59/2821924/b08a3668e23a/pone.0009238.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c59/2821924/bbd854f773a3/pone.0009238.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c59/2821924/df0e45b0cffd/pone.0009238.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c59/2821924/0e21885fe84c/pone.0009238.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c59/2821924/3d3544606a06/pone.0009238.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c59/2821924/c1d3d65a1a21/pone.0009238.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c59/2821924/b08a3668e23a/pone.0009238.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c59/2821924/bbd854f773a3/pone.0009238.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c59/2821924/df0e45b0cffd/pone.0009238.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c59/2821924/0e21885fe84c/pone.0009238.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c59/2821924/3d3544606a06/pone.0009238.g006.jpg

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