Department of Human Nutrition, Institute of Nutrition, Friedrich Schiller University, Jena, Germany.
Mol Nutr Food Res. 2010 May;54(5):588-98. doi: 10.1002/mnfr.200900457.
Vitamin E is known as the most important lipid antioxidant and is widely used to prevent age-associated diseases. Despite increasing knowledge about human vitamin E metabolism, little is known to justify its widespread use. As meta-analyses revealed even harmful effects of high vitamin E doses, a profound understanding of vitamin E metabolism is mandatory. By recent advances in analytical methodology, new metabolites with distinct physicochemical and biological properties were discovered. This review covers current methods to analyze vitamin E metabolites in biological samples. Special emphasis is laid on analytical applications for the identification and quantification of metabolites with a modified hydroxychromanol ring or a truncated side chain.
维生素 E 是一种重要的脂溶性抗氧化剂，被广泛用于预防与年龄相关的疾病。尽管人们对人类维生素 E 代谢的了解越来越多，但仍缺乏充分的理由来支持其广泛应用。由于荟萃分析显示高剂量维生素 E 甚至有有害作用，因此深入了解维生素 E 代谢是必要的。通过分析方法的最新进展，发现了具有独特理化性质和生物学特性的新代谢物。本综述介绍了目前分析生物样品中维生素 E 代谢物的方法。特别强调了用于鉴定和定量具有修饰的羟色醇环或截短侧链的代谢物的分析应用。
