School of Dentistry, Federal University of Uberlândia, Uberlândia, MG, Brazil.
Microsc Res Tech. 2010 Oct;73(11):1015-8. doi: 10.1002/jemt.20826.
The aim of this study was to evaluate the effects of radiotherapy in cortical bone channels network. Fourteen rabbits were divided in two groups and test group received single dose of 15 Gy cobalt-60 radiation in tibia, bilaterally. The animals were sacrificed and a segment of tibia was removed and histologically processed. Histological images were taken and had their bone channels segmented and called regions of interest (ROI). Images were analyzed through developed algorithms using the SCILAB mathematical environment, getting percentage of bone matrix, ROI areas, ROI perimeters, their standard deviations and Lacunarity. The osteocytes and empty lacunae were also counted. Data were evaluated using Kolmogorov-Smirnov, Mann Whitney, and Student's t test (P < 0.05). Significant differences in bone matrix percentage, area and perimeters of the channels, their respective standard deviations and lacunarity were found between groups. In conclusion, the radiotherapy causes reduction of bone matrix and modifies the morphology of bone channels network.
本研究旨在评估放射治疗对皮质骨通道网络的影响。14 只兔子被分为两组，实验组在双侧胫骨中接受单次 15 Gy 钴-60 辐射。动物被处死，取出一段胫骨并进行组织学处理。采集组织学图像，对骨通道进行分割并标记为感兴趣区域（ROI）。使用 SCILAB 数学环境开发的算法对图像进行分析，获得骨基质百分比、ROI 面积、ROI 周长及其标准差和空隙度。还计算了骨细胞和空骨陷窝的数量。使用 Kolmogorov-Smirnov、Mann-Whitney 和 Student's t 检验（P < 0.05）对数据进行评估。结果显示，两组间骨基质百分比、通道面积和周长及其标准差和空隙度存在显著差异。结论：放射治疗可导致骨基质减少，并改变骨通道网络的形态。
