电离辐射与骨质量：时相关效应。
Ionizing radiation and bone quality: time-dependent effects.
机构信息
Integrated Dental Clinic Program, Faculty of Dentistry, Federal University of Uberlândia, Avenida Pará s/n°, Campus Umuarama, Bloco 4L, Bairro Umuarama, Uberlândia, Minas Gerais, 38.400-902, Brazil.
Faculty of Dentistry, Federal University of Juiz de Fora, Faculdade de Odontologia - Campus Universitário - Bairro Martelos -, Juiz de Fora, Minas Gerais, 36036-300, Brazil.
出版信息
Radiat Oncol. 2019 Jan 22;14(1):15. doi: 10.1186/s13014-019-1219-y.
BACKGROUND
The aim of this study was to evaluate the ionizing radiation (IR) effects on rat bone 30 and 60 days after irradiation.
METHODS
Wistar rats were submitted to IR (30 Gy) on the left leg and were euthanized after 30 and 60 days. The legs were divided into four groups according to the treatment and euthanization time: C30 and C60 (right leg-without IR), IR30 and IR60 (left leg-with IR).
RESULTS
CT analysis showed more radiodensity in C60 compared with other groups, and IR60 showed more radiodensity than IR30. In histomorphometric analysis, C30 showed lower bone matrix values compared with IR30 and C60. Lacunarity analyses showed more homogeneous bone channel distribution in C30 than IR30. ATR-FTIR showed decrease in ratio of mature and immature crosslinks in IR30 compared with C30. Crystallinity Index was decrease in IR60 compared with C60. The Amide III + Collagen/HA ratio was increased in C60 compared with C30; however this ratio decreased in IR60 compared with IR30. Biomechanical analysis showed lower values in IR groups in both time.
CONCLUSIONS
IR damaged bone quality and decreased stiffness. Moreover, the results suggested that the deleterious effects of IR increased in the late time points.
背景
本研究旨在评估电离辐射（IR）对大鼠骨 30 和 60 天后的影响。
方法
Wistar 大鼠左腿接受 IR（30Gy）照射，照射后 30 和 60 天处死。根据处理和处死时间将腿分为四组：C30 和 C60（右腿-无 IR），IR30 和 IR60（左腿-IR）。
结果
CT 分析显示 C60 比其他组具有更高的放射密度，而 IR60 比 IR30 具有更高的放射密度。在组织形态计量学分析中，C30 与 IR30 和 C60 相比，骨基质值较低。Lacunarity 分析显示 C30 中骨通道分布更均匀。ATR-FTIR 显示 IR30 中成熟和未成熟交联的比例较 C30 降低。结晶度指数在 IR60 中较 C60 降低。酰胺 III + 胶原/HA 比值在 C60 中较 C30 增加；然而，IR60 中与 IR30 相比，该比值降低。生物力学分析显示 IR 组在两个时间点的数值均较低。
结论
IR 损伤了骨质量并降低了刚度。此外，结果表明 IR 的有害影响在后期时间点增加。
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