Fine A S, Egnor R W, Forrester E, Scopp I W, Stahl S S
J Invest Dermatol. 1978 Jan;70(1):16-20. doi: 10.1111/1523-1747.ep12543360.
Oral mucosal mitochondria were isolated and characterized morphologically by electron microscopy. Polarographic measurements were made of respiration and oxidative phosphorylation in the mitochondrial preparations. ADP:O ratios approaching or slightly exceeding the theoretical maxima and stabilized respiratory control ratios were achieved with malate + glutamate, succinate and ascorbate-N,N,N1N1 tetramethyl-p-phenylenediamine (TMPD) as substrates. Inhibition by rotenone, antimycin A, azide, and cyanide established the classical electron transport chain as the major pathway of mitochondrial respiration. Respiration of the oral mucosal mitochondria was stimulated by DNP in the presence of succinate. DNP-stimulated respiration exceeded that observed in the presence of ADP plus Pi and increasing the concentration of DNP progressively inhibited respiration.
分离口腔黏膜线粒体，并通过电子显微镜对其进行形态学表征。对线粒体制剂中的呼吸作用和氧化磷酸化进行极谱测量。以苹果酸 + 谷氨酸、琥珀酸以及抗坏血酸 - N,N,N',N'-四甲基 - p - 苯二胺（TMPD）作为底物时，ADP:O 比值接近或略超过理论最大值，并实现了稳定的呼吸控制率。鱼藤酮、抗霉素 A、叠氮化物和氰化物的抑制作用确定了经典电子传递链是线粒体呼吸的主要途径。在琥珀酸存在的情况下，DNP 刺激口腔黏膜线粒体的呼吸作用。DNP 刺激的呼吸作用超过了在 ADP 加 Pi 存在时观察到的呼吸作用，并且增加 DNP 的浓度会逐渐抑制呼吸作用。
