Department of Biology, Universidad Autónoma de Madrid, Madrid, Spain.
Syst Biol Reprod Med. 2010 Feb;56(1):27-36. doi: 10.3109/19396360903515430.
The aim of this study was to compare the rate of sperm DNA fragmentation (rSDF; increase of SDF over time) in fresh and gradient isolated frozen-thawed semen samples from male sperm donors of proven fertility. SDF was assessed in the two samples obtained from the same fifteen male donors after 0.5, 1.5, 4.5, 6, 24, 48, and 72 h of incubation in a humidified atmosphere of 5% CO(2) in air at 37 degrees C. Analysis was performed based on chromatin dispersion patterns evaluated using the Halosperm kit. No significant differences in SDF were obtained when fresh and gradient-isolated frozen-thawed spermatozoa were compared at baseline. However, the rSDF shown by the two samples differed and gradient-isolation selected for sperm subpopulations showing a lower variance for SDF. At the individual level, sperm selection by density gradient purification in frozen-thawed samples did not improve the levels of SDF when compared with the values obtained in fresh samples at baseline.
本研究旨在比较有生育能力的男性精子供体的新鲜和梯度分离冷冻解冻精液样本中精子 DNA 碎片化（rSDF；随时间推移 SDF 的增加）的比率。在 37°C 下于 5%CO(2)的加湿气氛中孵育 0.5、1.5、4.5、6、24、48 和 72 h 后，从同一 15 名男性供体获得的两个样本中评估 SDF。使用 Halosperm 试剂盒评估染色质弥散模式进行分析。在基线时，比较新鲜和梯度分离冷冻解冻的精子时，SDF 没有显著差异。然而，两个样本的 rSDF 不同，梯度分离选择了 SDF 变异性较低的精子亚群。在个体水平上，与新鲜样本基线相比，冷冻解冻样本中通过密度梯度纯化对精子的选择并不能提高 SDF 水平。
