用于淀粉酶的简单且易于接近的双荧光标记底物。
Simple and conveniently accessible bi-fluorescence-labeled substrates for amylases.
机构信息
Division of Material Science, Graduate School of Science and Engineering, Saitama University, Sakura, Saitama, Japan.
出版信息
Bioorg Med Chem Lett. 2010 Mar 15;20(6):1969-71. doi: 10.1016/j.bmcl.2010.01.117. Epub 2010 Jan 25.
PMID:20171095
Abstract
Synthesis of bi-fluorescence-labeled maltooligosaccharides for amylase assay was accomplished. Preliminary biological evaluation of both bi-fluorescence-labeled maltohexasaccharide and maltose using alpha-amylase was carried out, and the hexaosyl derivative showed unique variation on the basis of fluorescence resonance energy transfer (FRET).
摘要
双荧光标记麦芽寡糖的合成用于淀粉酶测定。利用α-淀粉酶对两种双荧光标记麦芽六糖和麦芽糖进行了初步的生物学评价，六糖衍生物基于荧光共振能量转移（FRET）显示出独特的变化。
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