Laboratory of Biochemistry A, Necker Hospital, Paris, France.
Biochem Biophys Res Commun. 2010 Mar 19;393(4):783-7. doi: 10.1016/j.bbrc.2010.02.082. Epub 2010 Feb 18.
We compared the apoptotic mechanism involved in U937 human monocytic cell line in presence of oxidized low-density lipoproteins (oxLDL) obtained after treatment with hypochlorous acid (HOCl) or copper (Cu). Both types of oxLDL induced U937 apoptotic cell death via the mitochondrial pathway. In contrast to HOCl-oxLDL, Cu-oxLDL induced apoptosis via a caspase-independent mechanism, with no activation of pro-caspase-3, but via the release of apoptosis inducing factor (AIF) from mitochondria. The apoptotic program of the monocyte differs depending on the mode of LDL oxidation, based on differences in the oxidatively modified components of the two oxLDL types.
我们比较了在次氯酸（HOCl）或铜（Cu）处理后获得的氧化型低密度脂蛋白（oxLDL）存在的情况下，U937 人单核细胞系中涉及的凋亡机制。两种类型的 oxLDL 均通过线粒体途径诱导 U937 凋亡细胞死亡。与 HOCl-oxLDL 相反，Cu-oxLDL 通过 caspase 非依赖性机制诱导凋亡，没有前胱冬酶-3 的激活，但通过凋亡诱导因子（AIF）从线粒体释放。单核细胞的凋亡程序因 LDL 氧化方式的不同而不同，这取决于两种 oxLDL 类型中氧化修饰成分的差异。
