Department of Microbiology and Immunology, Creighton University School of Medicine, 2500 California Plaza, Omaha, NE 68178, USA.
J Microbiol Methods. 2010 May;81(2):194-6. doi: 10.1016/j.mimet.2010.02.005. Epub 2010 Feb 18.
Our laboratory has developed a flow cytometric assay to quantify alveolar macrophage (Mcapital EF, Cyrillic) phagocytosis of bacteria within a live animal. Mcapital EF, Cyrillics collected by bronchoalveolar lavage from rats infected transtracheally with Syto 9-labeled bacteria are fluorescently labeled for identification and analyzed by flow cytometry to quantify their bacterial uptake.
我们实验室开发了一种流式细胞术检测方法，用于定量活体动物中肺泡巨噬细胞（Mcapital EF, Cyrillic）吞噬细菌的能力。Mcapital EF, Cyrillics 通过经气管感染 Syto 9 标记细菌的大鼠支气管肺泡灌洗收集，然后进行荧光标记以识别，并通过流式细胞术进行分析，以定量其细菌摄取量。
