Department of Chemistry, Pukyong National University, 599-1 Daeyon 3-dong, Busan, Republic of Korea.
Food Chem Toxicol. 2010 May;48(5):1243-9. doi: 10.1016/j.fct.2010.02.017. Epub 2010 Feb 18.
In this study, we examined protective effect of Sargassum thunbergii on reactive oxygen species (ROS) mediated oxidative stress in cellular systems. In addition, polyunsaturated fatty acids from S. thunbergii were identified and quantified by gas chromatography mass spectroscopy. Intracellular ROS levels were measured using a oxidation sensitive dye, 2',7'-dichlorofluorescein diacetate (DCFH-DA). Treatment with S. thunbergii significantly reduced intracellular ROS mediated cell damage and inhibited myeloperoxidase (MPO) activity assessed in tumor necrosis factor-alpha (TNF-alpha) stimulated human monocytic leukemia in a concentration-dependent manner. Moreover, antioxidative mechanisms by S. thunbergii were evaluated by measuring the expression levels of antioxidative enzymes such as superoxide dismutase (SOD-1), catalase, glutathione peroxidase and glutathione reductase. SOD-1 and glutathione reductase were up-regulated by S. thunbergii. Furthermore, S. thunbergii contains polyunsaturated fatty acids such as arachidonic acid, arachidic acid, palmitic acid, elaidic acid, linoleic acid, stearic acid and cis-5,8,11,14,17-eicosanoic acid. Therefore, these results suggested that S. thunbergii has nutraceutical effectiveness in prevention of ROS-induced tissue damage and potential natural antioxidant related to oxidative stress, which can be traceable to polyunsaturated fatty acids contained in S. thunbergii.
在这项研究中，我们研究了马尾藻对活性氧（ROS）介导的细胞系统氧化应激的保护作用。此外，通过气相色谱质谱法鉴定和定量了马尾藻中的多不饱和脂肪酸。通过氧化敏感染料 2'，7'-二氯荧光素二乙酸酯（DCFH-DA）测量细胞内 ROS 水平。马尾藻处理以浓度依赖性方式显著降低了细胞内 ROS 介导的细胞损伤，并抑制了肿瘤坏死因子-α（TNF-α）刺激的人单核白血病中的髓过氧化物酶（MPO）活性。此外，通过测量抗氧化酶如超氧化物歧化酶（SOD-1）、过氧化氢酶、谷胱甘肽过氧化物酶和谷胱甘肽还原酶的表达水平来评估马尾藻的抗氧化机制。马尾藻上调了 SOD-1 和谷胱甘肽还原酶。此外，马尾藻还含有多不饱和脂肪酸，如花生四烯酸、花生酸、棕榈酸、反油酸、亚油酸、硬脂酸和顺式-5,8,11,14,17-二十碳五烯酸。因此，这些结果表明马尾藻具有预防 ROS 诱导的组织损伤的营养功效，以及与氧化应激相关的潜在天然抗氧化剂，这可归因于马尾藻中含有的多不饱和脂肪酸。
