Piao Mei Jing, Yoon Weon Jong, Kang Hee Kyoung, Yoo Eun Sook, Koh Young Sang, Kim Dong Sam, Lee Nam Ho, Hyun Jin Won
School of Medicine, Jeju National University, Jeju 690-756, South Korea; E-Mails:
Int J Mol Sci. 2011;12(11):8146-60. doi: 10.3390/ijms12118146. Epub 2011 Nov 18.
The aim of this study was to investigate the cytoprotective properties of the ethyl acetate fraction of Sargassum muticum (SME) against ultraviolet B (UVB)-induced cell damage in human keratinocytes (HaCaT cells). SME exhibited scavenging activity toward the 1,1-diphenyl-2-picrylhydrazyl radicals and hydrogen peroxide (H(2)O(2)) and UVB-induced intracellular reactive oxygen species (ROS). SME also scavenged the hydroxyl radicals generated by the Fenton reaction (FeSO(4) + H(2)O(2)), which was detected using electron spin resonance spectrometry. In addition, SME decreased the level of lipid peroxidation that was increased by UVB radiation, and restored the level of protein expression and the activities of antioxidant enzymes that were decreased by UVB radiation. Furthermore, SME reduced UVB-induced apoptosis as shown by decreased DNA fragmentation and numbers of apoptotic bodies. These results suggest that SME protects human keratinocytes against UVB-induced oxidative stress by enhancing antioxidant activity in cells, thereby inhibiting apoptosis.
本研究的目的是调查鼠尾藻乙酸乙酯提取物（SME）对紫外线B（UVB）诱导的人角质形成细胞（HaCaT细胞）损伤的细胞保护特性。SME对1,1-二苯基-2-苦基肼自由基和过氧化氢（H₂O₂）以及UVB诱导的细胞内活性氧（ROS）具有清除活性。SME还清除了由芬顿反应（FeSO₄ + H₂O₂）产生的羟基自由基，这是通过电子自旋共振光谱法检测到的。此外，SME降低了UVB辐射增加的脂质过氧化水平，并恢复了UVB辐射降低的蛋白质表达水平和抗氧化酶活性。此外，SME减少了UVB诱导的细胞凋亡，表现为DNA片段化和凋亡小体数量减少。这些结果表明，SME通过增强细胞内抗氧化活性来保护人角质形成细胞免受UVB诱导的氧化应激，从而抑制细胞凋亡。
