人牙本质-牙髓复合体中的半胱氨酸组织蛋白酶。
Cysteine cathepsins in human dentin-pulp complex.
机构信息
Centro Interdisciplinar de Investigação Bioquímica, Universidade de Mogi das Cruzes, São Paulo, Brazil.
出版信息
J Endod. 2010 Mar;36(3):475-81. doi: 10.1016/j.joen.2009.12.034.
INTRODUCTION
Collagen-degrading matrix metalloproteinases (MMPs) are expressed by odontoblasts and present in dentin. We hypothesized that odontoblasts express other collagen-degrading enzymes such as cysteine cathepsins, and their activity would be present in dentin, because odontoblasts are known to express at least cathepsin D. Effect of transforming growth factor beta (TGF-beta) on cathepsin expression was also analyzed.
METHODS
Human odontoblasts and pulp tissue were cultured with and without TGF-beta, and cathepsin gene expression was analyzed with DNA microarrays. Dentin cathepsin and MMP activities were analyzed by degradation of respective specific fluorogenic substrates.
RESULTS
Both odontoblasts and pulp tissue demonstrated a wide range of cysteine cathepsin expression that gave minor responses to TGF-beta. Cathepsin and MMP activities were observed in all dentin samples, with significant negative correlations in their activities with tooth age.
CONCLUSIONS
These results demonstrate for the first time the presence of cysteine cathepsins in dentin and suggest their role, along with MMPs, in dentin modification with aging.
简介
牙本质中的破骨细胞表达胶原蛋白降解基质金属蛋白酶（MMPs）。我们假设成牙本质细胞表达其他胶原蛋白降解酶，如半胱氨酸组织蛋白酶，并且其活性存在于牙本质中，因为已知成牙本质细胞至少表达组织蛋白酶 D。还分析了转化生长因子 β（TGF-β）对组织蛋白酶表达的影响。
方法
用和不用 TGF-β 培养人成牙本质细胞和牙髓组织，并通过 DNA 微阵列分析组织蛋白酶基因表达。通过各自特异性荧光底物的降解分析牙本质中组织蛋白酶和 MMP 活性。
结果
成牙本质细胞和牙髓组织均表现出广泛的半胱氨酸组织蛋白酶表达，对 TGF-β 的反应较小。在所有牙本质样本中均观察到组织蛋白酶和 MMP 活性，其活性与牙齿年龄呈显著负相关。
结论
这些结果首次证明了半胱氨酸组织蛋白酶在牙本质中的存在，并表明它们与 MMP 一起，在牙本质随年龄的变化中起作用。
Zotero 插件