Department of Endodontics, Federal Fluminense University, Niterói, RJ, Brazil.
J Endod. 2010 Mar;36(3):515-9. doi: 10.1016/j.joen.2009.11.011. Epub 2010 Jan 25.
The biocompatibility of chelating agents and organic acids have been explained by a variety of methods, and suggestions for use have been based more on clinical observations and physicochemical properties than on biological aspects. The present study aimed to evaluate the inflammatory response of 17% EDTA, 17% EDTA-T, and 10% citric acid in bony defect created in rat jaws.
Mandibular through and through critical size defects were created bilaterally in 60 rats. Fibrinol (Baldacchi SA, São Paulo, Brazil), a cube-shaped compound of absorbable bovine fibrin foam and sodium chloride, was used as a carrier of the substances. One side had received Fibrinol (control), whereas the opposite side had received Fibrinol soaked with each substance on the 1st, on the 7th, on the 14th, and on the 28th day (n=5 for each day). Hemijaws were prepared for light microscopy, and samples were stained with hematoxylin and eosin. Digitized images were analyzed with a morphometric software (ImageJ; National Institute of Mental Health, Bethesda, MD). to obtain the number of inflammatory cells per area. Comparisons were performed by using the Kruskal-Wallis test (p=0.05).
For all days, 10% citric acid and 17% EDTA-T showed, respectively, the lowest and highest number of inflammatory cells per area. All tested substances and controls showed the highest inflammatory cell response on the 14th day.
Among the tested substances, 10% citric acid proved to be the less aggressive tested solution at 14 days. At 28 days, all solutions were similar, but EDTA-T kept showing the higher number of inflammatory cells.
螯合剂和有机酸的生物相容性已经通过多种方法得到了解释，其使用建议更多地基于临床观察和物理化学性质，而不是生物学方面。本研究旨在评估 17% EDTA、17% EDTA-T 和 10%柠檬酸在大鼠颌骨骨缺损中的炎症反应。
在 60 只大鼠的双侧下颌骨中创建贯穿性临界尺寸缺损。Fibrinol（Baldacchi SA，圣保罗，巴西）是一种可吸收牛纤维蛋白泡沫和氯化钠的立方化合物，用作物质的载体。一侧接受 Fibrinol（对照），而另一侧在第 1、第 7、第 14 和第 28 天接受 Fibrinol 浸泡的每种物质（每天 n=5）。制备半颌进行光镜检查，并用苏木精和伊红染色。使用形态计量学软件（ImageJ；美国国立精神卫生研究所，贝塞斯达，MD）对数字化图像进行分析，以获得每个区域的炎症细胞数量。使用 Kruskal-Wallis 检验（p=0.05）进行比较。
在所有天数中，10%柠檬酸和 17% EDTA-T 分别显示每个区域的炎症细胞数量最低和最高。所有测试物质和对照在第 14 天表现出最高的炎症细胞反应。
在所测试的物质中，10%柠檬酸在第 14 天被证明是最不具侵袭性的测试溶液。在第 28 天，所有溶液都相似，但 EDTA-T 仍然显示出更高数量的炎症细胞。
