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牙齿组织工程：用于天然支架的牙齿去细胞化

Tooth tissue engineering: tooth decellularization for natural scaffold.

作者信息

de Sousa Iwamoto Luciana Aparecida, Duailibi Monica Talarico, Iwamoto Gerson Yoshinobu, Juliano Yara, Duailibi Michel Silvio, Ossamu Tanaka Francisco André, Duailibi Silvio Eduardo

机构信息

CTCMol, Center of Cellular & Molecular Therapy, UNIFESP- Universidade Federal de Sao Paulo- Escola Paulista de Medicina, Sao Paulo, Brazil; Translational Surgery, Surgery Department, UNIFESP- Universidade Federal de Sao Paulo- Escola Paulista de Medicina, Sao Paulo, Brazil; CTCMol, Center of Cellular & Molecular Therapy, UNIFESP- Universidade Federal de Sao Paulo- Escola Paulista de Medicina, Sao Paulo, Brazil; Translational Surgery, Surgery Department, UNIFESP- Universidade Federal de Sao Paulo- Escola Paulista de Medicina, Sao Paulo, Brazil.

CTCMol, Center of Cellular & Molecular Therapy, UNIFESP- Universidade Federal de Sao Paulo- Escola Paulista de Medicina, Sao Paulo, Brazil; Translational Surgery, Surgery Department, UNIFESP- Universidade Federal de Sao Paulo- Escola Paulista de Medicina, Sao Paulo, Brazil; National Institute of Science & Technology, Biofabrication Institute, BIOFABRIS, Campinas, São Paulo, Brazil; CTCMol, Center of Cellular & Molecular Therapy, UNIFESP- Universidade Federal de Sao Paulo- Escola Paulista de Medicina, Sao Paulo, Brazil; Translational Surgery, Surgery Department, UNIFESP- Universidade Federal de Sao Paulo- Escola Paulista de Medicina, Sao Paulo, Brazil; National Institute of Science & Technology, Biofabrication Institute, BIOFABRIS, Campinas, São Paulo, Brazil.

出版信息

Future Sci OA. 2016 Mar 31;2(2):FSO121. doi: 10.4155/fsoa-2016-0016. eCollection 2016 Jun.

DOI:10.4155/fsoa-2016-0016
PMID:28031968
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5137886/
Abstract

AIM

Tissue engineering is a multidisciplinary science that aims to produce replacement organs and biological substitutes. One of the techniques involves decellularizing a biological organ without altering its structure. One challenge is how to demonstrate which method would be better for this process.

METHODOLOGY

Fifty premolar teeth were divided into five groups: G1 (control): solution of 10% formaldehyde; G2: phosphate buffer saline (PBS), 28 g of tetrasodium ethylenediaminetetraacetic (EDTA), sodium hypochlorite 2.5% (SH); G3: PBS, EDTA and 40v hydrogen peroxide (HP); G4: PBS, EDTA, SH, enzymatic detergent (ED); and G5: PBS, EDTA, HP, ED. Each group was analyzed by scanning electron microscopy (SEM), x-ray, measured weights and color and received statistical analysis.

CONCLUSION

This study demonstrated that G5 was the most appropriate method to obtain a natural scaffold.

摘要

目的

组织工程是一门多学科科学，旨在制造替代器官和生物替代品。其中一种技术涉及在不改变生物器官结构的情况下使其脱细胞。一个挑战是如何证明哪种方法更适合此过程。

方法

50颗前磨牙被分为五组：G1（对照组）：10%甲醛溶液；G2：磷酸盐缓冲盐水（PBS）、28克乙二胺四乙酸四钠（EDTA）、2.5%次氯酸钠（SH）；G3：PBS、EDTA和40体积分数的过氧化氢（HP）；G4：PBS、EDTA、SH、酶洗涤剂（ED）；G5：PBS、EDTA、HP、ED。每组通过扫描电子显微镜（SEM）、X射线进行分析，测量重量和颜色，并进行统计分析。

结论

本研究表明，G5是获得天然支架的最合适方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ee0/5137886/5776217be4e4/fsoa-02-121-g5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ee0/5137886/3ec781ee1052/fsoa-02-121-g1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ee0/5137886/3ea76afad40d/fsoa-02-121-g2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ee0/5137886/daa44305ae1b/fsoa-02-121-g3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ee0/5137886/41ead42dd53e/fsoa-02-121-g4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ee0/5137886/5776217be4e4/fsoa-02-121-g5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ee0/5137886/3ec781ee1052/fsoa-02-121-g1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ee0/5137886/3ea76afad40d/fsoa-02-121-g2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ee0/5137886/daa44305ae1b/fsoa-02-121-g3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ee0/5137886/41ead42dd53e/fsoa-02-121-g4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ee0/5137886/5776217be4e4/fsoa-02-121-g5.jpg

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